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AssigneeUniversity of Central Florida Research Foundation, Inc.
InventorsPappachan Kolattukudy, Tatiana Sirakova, Jaiyanth Daniel, Chirajyoti Deb
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A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.
A method of inducing latency in Mycobacterium, the method comprising growing a pure culture of Mycobacterium exposed to a plurality of stress conditions, the stress conditions selected from a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide, a relatively low gas phase oxygen level, and combinations thereof.
A method of inducing a pure culture of Mycobacterium to become rifampicin resistant and to store an increased lipid content, the method comprising growing the culture simultaneously exposed to multiple stress conditions, the stress conditions including at least a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level.
An in vitro model of latent tuberculosis, the model comprising an isolated culture of THP1 derived macrophages containing ingested Mycobacterium tuberculosis bacteria and incubated under hypoxic conditions for a time sufficient for the bacteria to accumulate increased lipids therein.
An in vitro model of latent mycobacterial infection, the model comprising an isolated culture of THP1 derived macrophages containing ingested Mycobacterium spp. cells and incubated under hypoxic conditions for a time sufficient for the bacteria to accumulate increased lipids therein.
An in vitro model of mycobacterial infection, the model comprising an isolated culture of THP1 derived macrophages containing ingested Mycobacterium spp. cells.
A method of making a model of latent tuberculosis, the method comprising:
A method of making a model of mycobacterial infection, the method comprising:
Dependencies are inferred from the claim text.