Solution for the indefinite maintenance of nucleic acids in the cell of origin thereof

Abstract

The invention relates to a product having characteristics which enable a biological sample obtained from an individual to be maintained under conditions such that the quality of nucleic acids later extracted is not affected, thereby guaranteeing the integrity of the sample regardless of the time that elapses between the sample being obtained and the nucleic acid being extracted. A solid (organic) or liquid (blood, semen, cell culture) biological sample from a eukaryote or prokaryote is mixed in a solution comprised of a mixture of trisodium ethylenediaminetetraacetic acid (EDTA), sodium fluoride (NaF), and saturation with thymol (extracted from Thymus vulgaris L.) in order to prevent contamination and bacterial growth. The solution is adjusted to a pH of 8.0. The solution can be used to collect samples and to maintain the samples at ambient temperature for an indefinite period for the later extraction of nucleic acids and PCR, cloning, sequencing, hybridization or mutagenesis with sufficient integrity and reliability.

Dependencies are inferred from the claim text.