Nucleic acid detecting cassette and nucleic acid detecting apparatus
OwnerSadato Hongo, Takahiro Kokubo +3inventors
InventorsSadato Hongo, Takahiro Kokubo, Hideki Horiuchi, Koji Hashimoto, Jun Okada
Life of this patent
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Abstract
In a nucleic acid detecting cassette in which a heating treatment chamber and a liquid delivery channel are constituted of a stationary member and flexible members, the heating treatment chamber is held by convex heating sections from both sides, whereby the heat treatment chamber and the liquid delivery channel are spatially separated from each other. Thus, it is possible to automatically execute a consistent process including nucleic acid amplification and other required treatments of the object sample as well as detection of a target nucleic acid.
Claims31
A nucleic acid detecting cassette comprising:
- a plate-like member which includes a front surface and an opposite surface opposed to the front surface, is made of a rigid material, and further includes a first through-hole, a second through-hole, a first groove formed in the front surface, a first groove formed in the front surface, and a second groove formed in the opposite surface;
- a first and second sheet-like members which cover the front surface and the opposite surface, respectively;
- a pump section formed in the plate-like member, for supplying air pressure;
- a sample chamber section which is defined when the first through-hole is closed by the first and second sheet-like members, communicates with the pump section, and holds a nucleic acid sample therein;
- a detection section which is formed in the plate-like member, communicates with the sample chamber section, receives the nucleic acid sample, and detects a target nucleic acid from the nucleic acid sample;
- a return channel for making the detection section and the pump section communicate with each other;
- a first channel defined when the first groove is covered with the first sheet-like member;
- a first channel defined when the first groove is covered with the first sheet-like member;
- a second channel defined when the second groove is covered with the second sheet-like member;
- a third channel defined when the second through-hole is covered with the first sheet-like member and the second sheet-like member, wherein
- the pump section, the sample chamber section, and the detection section communicate with each other in a circular form,
- the first channel and the second channel are connected to the sample chamber section,
- the third channel is connected to the second channel,
- the first channel is connected to the third channel,
- air pressure supplied by the pump section is applied to the sample chamber section through the first channel, whereby the nucleic acid sample held in the sample chamber section is discharged through the second channel, the third channel, and the first channel, and
- the air pressure returns from the detection section to the pump section through the circular flow path.
Dependencies are inferred from the claim text.