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AssigneeOrion Diagnostica Oy
InventorsHenri Rainer Nordlund, Olli Heikki Laitinen, Vesa Pekka Hytonen, Markku Sakari Kulomaa
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Two circularly permuted avidin monomers are designed. The circularly permuted monomers (cpAvd5→4 and cpAvd6→5) are fused and the resulting fusion peptides (dcAvd) form a pseudo-tetrameric dual-chain avidin, which is biologically active in biotin binding and shows similar structural characteristics as wild-type avidin. The dcAvd makes the development of dual-affinity avidins possible by allowing the adjustment of the ligand binding properties in the half of the binding sites differently than in the rest of the sites. The present invention provides further a single-chain avidin (scAvd) where two dcAvd-molecules are fused together via a linker to form a single polypeptide with four binding sites for biotin.
A circularly permuted avidin monomer, wherein the carboxyl terminal amino acid and the amino terminal amino acid of the polypeptide of the avidin monomer have been joined directly or via a linker, and new carboxyl and amino termini have been created by cleavage of the polypeptide and the resulting circularly permuted avidin monomer binds biotin or other ligand.
A dual-chain pseudo-tetrameric avidin, wherein said dual-chain pseudo-tetrameric avidin comprises two dual-chain avidins (dcAvd).
Dependencies are inferred from the claim text.