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AssigneeSeoul National University Industry Foundation
InventorsUhTaek Oh, Byung Moon Kim, Seung Pyo Park, Heung Sik Na
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The present invention relates to a method for producing a recombinant, spider toxin peptide and analgesic compositions containing said peptide. More specifically, the present invention relates to a method in which the gene for GsMTx4 is subcloned into a vector, so that it is linked to a secretion signal sequence of the alpha factor and under the control of methanol-inducible alcohol oxidase (AOX) promoter to construct a recombinant yeast expression plasmid. Yeast cells are transformed with this plasmid to produce the GsMTx4 peptide and analgesic compositions containing said peptide. The recombinant yeast expression system of the present invention affords a more stable method for producing GsMTx4 than its natural route. Thus the GsMTx4 peptide and its derivatives produced by the method of this invention can be used in the cure of related diseases such as heart failure as the peptide specifically inhibits mechanosensitive ion channels.
A gene construct containing a polynucleotide encoding the amino acid sequence for the GsMTx4 peptide, a signal sequence for secretion and a methanol-assimilating promoter for expression in yeasts.
Dependencies are inferred from the claim text.