Assays and methods for determining stim2 activity
AssigneeThe Queen's Medical Center
InventorsReinhold PENNER, Andrea Fleig
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Abstract
The present invention provides assays and methods for determining levels of STIM2 activity, thus providing tools for the characterization and study of the regulation of intracellular calcium levels.
Claims8
An assay for STIM2 activity in a cell comprising measuring Icrac activity, wherein an increase in Icrac activity in response to application of 2-APB in the absence of calcium store depletion indicates a level of STIM2 activity in said cell.
An assay for STIM2 activity in a cell comprising measuring Icrac activity after application of an aminoglycoside antibiotic, wherein suppression of Icrac activity by said antibiotic indicates a level of STIM2 activity in said cell.
An assay for agents that modulate STIM2 activity comprising measuring Icrac activity in response to application of 2-APB in the absence of calcium store depletion, wherein an agent that modulates said Icrac activity is identified as an agent that modulates STIM2 activity.
Dependencies are inferred from the claim text.