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US 2009/0023174 A1Published application
Methods and compositions for detecting immune responses
AssigneeGovernment of the United States of America, represented by the Secretary, Department of Health
InventorKelledy Manson
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Abstract
Methods and compositions for detecting immune responses and antigen-specific cells are described herein.
Claims35
A method for detecting an antigen-specific hematopoietic cell in a biological sample, the method comprising:
- (a) providing a biological sample comprising a hematopoietic cell of a first species;
- (b) providing a target cell of a second species, wherein the target cell comprises an antigen;
- (c) contacting the target cell with the sample;
- and
- (d) detecting an immune activation marker or activity in the sample, wherein an increase in expression of the immune activation marker or activity in the sample, relative to a control, is an indication that the sample comprises an antigen-specific hematopoietic cell;
- wherein a cell essentially identical to the target cell but lacking the antigen does not stimulate expression of the immune activation marker or activity in hematopoietic cells of the first species;
- and wherein the first and second species can be the same or different.
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A method of evaluating treatment of a subject, the method comprising:
- (a) obtaining a sample comprising hematopoietic cells from a subject of a first species, wherein the subject is undergoing or being evaluated for an immunotherapeutic treatment for a disease or condition;
- (b) providing a target cell of a second species, wherein the target cell comprises an antigen;
- (c) contacting the target cell with the sample;
- (d) detecting an immune activation marker or activity in the sample, wherein an increase in expression of the immune activation marker or activity in the sample, relative to a control, is an indication that the sample comprises an antigen-specific hematopoietic cell;
- wherein a cell essentially identical to the target cell but lacking the antigen does not stimulate expression of the immune activation marker or activity in hematopoietic cells of the first species, thereby evaluating treatment of the subject or evaluating the subject;
- and wherein the first and second species can be the same or different.
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Dependencies are inferred from the claim text.