High throughput sarcomeric assay

A method of identifying a candidate agent as a modulator of function of a target protein complex wherein said target protein complex comprises a biochemically functional sarcomere and said method comprises:

• a) adding a candidate agent to a mixture comprising a target protein complex that directly or indirectly produces ADP or phosphate under conditions which normally allow the production of ADP or phosphate;
• b) subjecting the mixture to an enzymatic reaction that uses said ADP or phosphate as a substrate under conditions which normally allow the ADP or phosphate to be utilized;
• and
• c) determining the level of activity of the enzymatic reaction wherein a change in said level between the presence and absence of said candidate agent indicates a modulator of said target protein function.