Methods for Detecting Minimum Residual Disease
AssigneeSloan-Kettering Institute for Cancer Research
InventorNai-Kong Cheung
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Abstract
The present invention features methods and compositions for identifying markers of minimum residual disease (MRD), as well as markers of metastatic cells. The present invention further provides methods for detecting MRD and metastatic cell in a subject.
Claims37
A method for identifying a marker of minimum residual disease (MRD) comprising:
- a) measuring the mRNA expression level of a gene in a tumor;
- b) measuring the mRNA expression level of the gene in the bone marrow or peripheral blood from normal subjects or subjects cured of their tumor;
- and
- c) comparing the mRNA expression level in (a) to the mRNA level in (b),
- wherein a gene with an (a)/(b) ratio of at least about 20 for marrow or at least about 5 for blood is identified as a marker of MRD.
A method for identifying a marker of circulating tumor cells in blood or bone marrow comprising:
- a) measuring the mRNA expression level of a gene in a tumor;
- b) measuring the mRNA expression level of the gene in the peripheral blood or the bone marrow;
- and
- c) comparing the mRNA expression level in (a) to the mRNA level in (b), ps wherein a gene with an (a)/(b) ratio of at least about 5 is identified as a marker of circulating tumor cells in the blood or in the bone marrow.
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A method of identifying a subject with MRD comprising measuring the mRNA expression level of at least one marker of MRD in a peripheral blood or bone marrow sample obtained from a subject in remission from a tumor, wherein expression of the MRD marker in the peripheral blood or bone marrow sample identifies the subject as having MRD.
A method of identifying a metastatic tumor cell in a subject comprising detecting mRNA expression of at least one marker of MRD in a peripheral blood or bone marrow sample obtained from the subject, wherein expression of the MRD marker in the peripheral blood or bone marrow sample identifies the subject as having a metastatic tumor cell.
A method for identifying a single tumor cell in the blood of a subject comprising detecting mRNA expression of at least one marker of MRD in a blood sample obtained from the subject, wherein expression of the MRD marker in the blood sample of the subject identifies a single tumor cell in the blood of the subject.
A method for identifying a single tumor cell in the bone marrow of a subject comprising detecting mRNA expression of at least one marker of MRD in a bone marrow sample obtained from the subject, wherein expression of the MRD marker in the bone marrow sample of the subject identifies a single tumor cell in the bone marrow of the subject.
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Dependencies are inferred from the claim text.