Method for detecting bacteria of the genus mycobacterium (acid-fast bacteria) and kit for the same

A method for identifying one of Mycobacterium tuberculosis, Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium kansasi, and which comprises performing a nucleic acid amplification reaction using at least 2 primers which are selected from the following (a) to (d):

• (a) a primer for nucleic acid amplification that is a nucleotide sequence of at least 15 or more continuous nucleotides contained in the nucleotide sequence represented by SEQ ID NO:
• 3 and comprises a nucleotide sequence containing at least 3 or more nucleotides consisting of G (the nucleotide 26) and the following nucleotides on the 3′ side in SEQ ID NO:
• 3;
• (b) a primer for nucleic acid amplification that is a nucleotide sequence of at least 15 or more continuous nucleotides contained in the nucleotide sequence represented by SEQ ID NO:
• 6 and comprises a nucleotide sequence containing at least 3 or more nucleotides consisting of G (the nucleotide 26) and the following nucleotides on the 3 side in SEQ ID NO:
• 6;
• (c) a primer for nucleic acid amplification that is a nucleotide sequence of at least 15 or more continuous nucleotides contained in the nucleotide sequence represented by SEQ ID NO:
• 9 and comprises a nucleotide sequence containing at least 3 or more nucleotides consisting of G (the nucleotide 26) and the following nucleotides on the 3′ side in SEQ ID NO:
• 9;
• and
• (d) a primer for nucleic acid amplification that is a nucleotide sequence of at least 15 or more continuous nucleotides contained in the nucleotide sequence represented by SEQ ID NO:
• 25 and comprises a nucleotide sequence containing at least 3 or more nucleotides consisting of G (the nucleotide 26) and the following nucleotides on the 3′ side in SEQ ID NO:
• 25;
• and differentiating bacterial species based on the presence or the absence of amplification reaction.