US 2009/0023140 A1 · CROSSLINKING AGENT, CROSSLINKING METHOD, METHOD OF CONTROLLING GENE EXPRESSION, AND METHOD OF EXAMINING GENE FUNCTION

Abstract

The present invention provides a crosslinking agent which have photodegradable protective groups at two ends to crosslink double-stranded nucleic acid, a nucleic acid and a protein or a polypeptide, or proteins or polypeptides, in particular, double-stranded RNA; a method for crosslinking a double-stranded RNA or the like using the same; a method for regulating gene expression, which can control the expression of a target gene at an arbitrary timing and location; and a method for examining a gene function. According to the present invention, crosslinking between double-stranded nucleic acids between a nucleic acid and a protein or a polypeptide, or between proteins or polypeptides, in particular, between double-stranded RNA can be easily formed, and in addition, the crosslinking can also be easily removed, so that the expression of a target gene can be easily controlled at an arbitrary timing and location with high efficiency. Hence, as a result, function examination and/or identification of a gene that is expressed at a specific timing and location can be performed. In addition, the RNAi effect of a double-stranded RNA (siRNA) that cannot be easily inhibited by a conventional caged compound can be inhibited, and the expression of a target gene can be easily controlled at an arbitrary timing and location.

Claims15

Dependent claims

A compound represented by the following general formula (1):

Q1-A1-T1-A2-Q2 (1)
(where Q1 and Q2 each independently represent a photodegradable protective group;
A1 and A2 each independently represent an alkylene group, —O—, —NR1—, —O—CO—, —CO—O—, —C—O—C—, —NR2—COO—, —OCO—NR2—, —NR3—CO—, —CO—NR3—, or —O—COO— (R1 to R3 each independently represent hydrogen or an alkyl group);
T1 represents an alkylene group, an arylene group, an aralkylene group, an alkylene group containing a hetero atom, an arylene group containing a hetero atom, or an aralkylene group containing a hetero atom).

1 more element

Dependencies are inferred from the claim text.

Crosslinking agent, crosslinking method, method of controlling gene expression, and method of examining gene function

Life of this patent

Crosslinking agent, crosslinking method, method of controlling gene expression, and method of examining gene function

Life of this patent

Abstract

Claims15