Circuit arrangement for injecting nucleic acids and other biologically active molecules into the nucleus of higher eucaryontic cells using electrical current

A method for introducing nucleic acids, peptides, proteins and/or other biologically active molecules into the cell nucleus of eukaryotic cells by means of electric current or for the treatment of cells, cell derivatives, subcellular particles and/or vesicles with electric current, said method comprising:

• supplying at least one voltage pulse to the cells, cell derivatives, subcellular particles and/or vesicles,
• measuring the quantity of charge supplied by the voltage pulse in at least one selectable time interval, wherein a preset desired quantity of charge is compared with the actually supplied quantity of charge, and
• terminating the voltage pulse on reaching or exceeding the desired quantity of charge.