US 2009/0022873 A1Published application

Genes Useful for the Industrial Production of Citric Acid

AssigneeAdcuram Nutrition Holding GmbH

InventorsHugo Marc Karel Bauweleers, Dominique Robert Groeseneken, Nicolaas Maria Elisabeth Peij

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Abstract

The present invention relates to newly identified genes that encode proteins that are involved in the (bio)synthesis of citric acid. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of citric acid from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for production of citric acid.

Claims32

Dependent claims

A TS08 and/or TS09 polynucleotide selected from the group consisting of:

(a) polynucleotides encoding a TS08 or TS09 polypeptide comprising an amino acid sequence according to SEQ ID NO:
2 or SEQ ID NO:
7, respectively;
(b) polynucleotides comprising a nucleotide sequence according to SEQ ID NO:
1 or SEQ ID NO:
6;
(c) polynucleotides comprising a nucleotide sequence obtainable by nucleic acid amplification such as polymerase chain reaction, using genomic DNA from a microorganism as a template and a primer set according to SEQ ID NO:3 and SEQ ID NO:4, or SEQ ID NO:8 and SEQ ID NO:9, respectively;
(d) polynucleotides comprising a nucleotide sequence encoding a fragment or derivative of a polypeptide encoded by a polynucleotide of any of (a) to (c) wherein in said derivative one or more amino acid residues are conservatively substituted compared to said polypeptide, and said fragment or derivative has the activity of a TS08 or TS09 polypeptide;
(e) polynucleotides the complementary strand of which hybridizes under stringent conditions to a polynucleotide as defined in any one of (a) to (d) and which encode a TS08 or TS09 polypeptide;
(f) polynucleotides which are at least 70%, such as 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98 or 99% homologous to a polynucleotide as defined in any one of (a) to (d) and which encode a TS08 or TS09 polypeptide, or the complementary strand of such a polynucleotide.

7 more elements

Dependencies are inferred from the claim text.