Surface proteins of streptococcus pyogenes
AssigneeWyeth
InventorsStephen Bruce Olmsted, Robert John Zagursky, Elliott Bruce Nickbarg, Laurie Anne Winter
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Abstract
β-hemolytic streptococci polynucleotides, polypeptides, particularly Streptococcus pyogenes polypeptides and polynucleotides, and antibodies of these polypeptides are described. The polynucleotides, polypeptides, and antibodies of the invention can be formulated for use as immunogenic compositions. Also disclosed are methods for immunizing against and reducing β-hemolytic streptococcal infection, and for detecting β-hemolytic streptococci in a biological sample.
Claims47
An isolated polypeptide comprising:
- (a) an amino acid sequence that has at least 70% identity to the amino acid sequence of SEQ ID NO:48;
- or
- (b) an amino acid sequence that is encoded by a nucleic acid sequence having at least 70% identity to the nucleic acid sequence of SEQ ID NO:47;
- wherein administration of the isolated polypeptide induces antibodies having opsonophagocytic activity of at least about 30 percent killing of bacteria as measured by decrease in colony forming units (CFU) in OPA versus a negative control.
An isolated polypeptide comprising:
- (a) an amino acid sequence that comprises the amino acid sequence of SEQ ID NO:48;
- or
- (b) an amino acid sequence that is encoded by a nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO:47.
An isolated polypeptide comprising:
- an amino acid sequence that comprises at least 7 contiguous amino acid residues of the amino acid sequence of SEQ ID NO:48;
- wherein administration of the isolated polypeptide induces antibodies having opsonophagocytic activity of at least about 30 percent killing of bacteria as measured by decrease in colony forming units (CFU) in OPA versus a negative control.
An isolated polynucleotide comprising:
- (i) a nucleotide sequence that encodes an amino acid sequence that has at least 70% identity to the amino acid sequence of SEQ ID NO:48;
- or
- (b) a nucleotide sequence that has at least 70% identity to the nucleic acid sequence of SEQ ID NO:47;
- wherein the isolated polynucleotide encodes a polypeptide that exhibits opsonophagocytic activity of at least about 30 percent killing of bacteria as measured by decrease in colony forming units (CFU) in OPA versus a negative control.
An isolated polynucleotide comprising:
- (a) a nucleotide sequence that comprises the nucleic acid sequence of SEQ ID NO:47;
- or
- (b) a nucleotide sequence that encodes an isolated polynucleotide comprising the amino acid sequence of SEQ ID NO:48.
An immunogenic composition comprising:
- (i) an isolated polypeptide that is substantially conserved across strains of β-hemolytic streptococci and that is effective in preventing or ameliorating a β-hemolytic streptococcal colonization or infection in a susceptible subject, said isolated polypeptide having at least 70% identity to the amino acid sequence of SEQ ID NO:48;
- or
- (ii) an immunogenic fragment of (i).
An isolated polypeptide comprising:
- (i) an amino acid sequence that has at least 70% identity to an amino acid sequence of any of even numbered SEQ ID NOS:
- 2-668;
- (ii) an amino acid sequence of any of even numbered SEQ ID NOS:
- 2-668;
- (iii) an immunogenic fragment of any amino acid sequence of (i) or (ii);
- (iv) at least 7 contiguous amino acid residues of any amino acid sequence of (i) or (ii);
- or
- (v) a biological equivalent of any of (i), (ii), (iii) or (iv) that is effective for preventing or ameliorating β-hemolytic streptococcal colonization or infection in a susceptible subject.
Dependencies are inferred from the claim text.