Method forisolating stem cells from cryopreserved dental tissue

Abstract

The invention relates to a method for isolation of multipotent stem cells from dental tissue in which the stem cells are extracted from a tissue structure and then cultured. The invention also relates to stem cells isolated by means of the method according to the invention as well as bone cells and nerve cells produced by means of the method according to the invention. The invention also relates to a method for producing a bank of stem cells in which the cells are stored by means of the method according to the invention. According to the present invention, the cells of a pad-like soft tissue that can be localized beneath the papilla directly on the apical side of an extracted immature tooth are cryopreserved in the tissue structure such that the tissue structure is disintegrated to extract the stem cells only after thawing. These results show that stem cells/progenitor cells can be isolated even after cryopreservation of the source tissue and these stem cells/progenitor cells respond to osteogenic stimulation. In addition, the response of cells after cryopreservation turns out to be stronger than that without cryopreservation.

Dependencies are inferred from the claim text.