Bacterial delivery of biologically active polypeptides
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsRobert John Moore, Julian Ian Rood, Scott Andrew Sheedy
Life of this patent
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Abstract
The present invention relates to methods for the delivery of biologically active polypeptides to a subject by colonising non-pathogenic Gram negative bacteria. Also provided by this invention are methods of treating or preventing diseases by administering colonising Gram negative bacteria that express one or more biologically active polypeptides. The colonising non-pathogenic Gram negative bacteria may be administered in pharmaceutical formulations.
Claims26
1-54. (canceled)
A method of delivering one or more heterologous biologically active polypeptides to a subject, the method comprising administering to the subject a colonising non-pathogenic Gram negative bacterium that expresses one or more heterologous biologically active polypeptides.
A method of identifying a colonising non-pathogenic Gram negative bacterial strain, the method comprising:
- i) isolating one or more Gram negative bacterial strain from a subject;
- ii) marking the one or more Gram negative bacterial strain;
- iii) re-introducing the one or more marked Gram negative bacterial strain into the subject;
- and
- iv) determining whether the one or more Gram negative bacterial strain colonises said subject.
A method of treating or preventing a disease in a subject, the method comprising administering to the subject a therapeutically effective amount of a colonising non-pathogenic Gram negative bacterium that expresses one or more heterologous biologically active peptides.
A colonising non-pathogenic Gram negative bacterium that expresses one or more heterologous biologically active polypeptides, which when administered to a subject, colonises said subject.
A colonising non-pathogenic Gram negative bacterium selected from CCEC22 as deposited with the National Measurement Institute (formerly AGAL) under accession number NM05/45635, CCEC31 as deposited with the National Measurement Institute (formerly AGAL) under accession number NM05/45636 or CCEC59 as deposited with the National Measurement Institute (formerly AGAL) under accession number NM05/45637.
Dependencies are inferred from the claim text.