Methods for identifying treatments that treat and/or prevent UV irradiation induced photoaging
AssigneeThe Regents of the University of Michigan
InventorsGary J. Fisher, Yiru Xu, Sewon Kang, John J. Voorhees
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Abstract
Methods are provided for ascertaining and measuring RPTP-κ activity in response to insults such as UV irradiation and with respect to administration of a treatment and/or composition. Attenuation of EGFR activity by RPTP-κ affects aspects of photoaging, including damage to the skin, suppression of the immune system, DNA damage, and connective tissue degradation. Intervention with respect to the effects of photoaging can include protection of RPTP-κ from oxidation. The methods can be used for discovery of anti-aging treatments, adjuncts, or other preventative treatments, such as sunscreens.
Claims39
A method for measuring UV irradiation based inhibition of receptor type protein-tyrosine phosphatase kappa (RPTP-κ) activity comprising:
- irradiating RPTP-κ with UV;
- and
- measuring the activity of RPTP-κ.
A method for measuring whether a treatment affects RPTP-κ activity in a cell, comprising:
- providing cells expressing RPTP-κ;
- applying the treatment to at least one cell;
- exposing treated and untreated cells to UV irradiation;
- measuring the activity of RPTP-κ, wherein a difference in the activity of RPTP-κ following UV irradiation in the treated and untreated cells is indicative of the treatment affecting RPTP-κ activity.
A method for determining whether an insult affects RPTP-κ activity, comprising:
- providing cells expressing RPTP-κ;
- measuring initial RPTP-κ activity;
- exposing the cells to the insult;
- measuring RPTP-κ activity after exposure to the insult;
- and
- identifying the insult as affecting RPTP-κ activity if the RPTP-κ activity measured after exposure to the insult is different than the RPTP-κ activity prior to the insult.
A method for determining whether a composition protects RPTP-κ activity from an insult that increases EGFR phosphorylation, comprising:
- providing a cell having a known RPTP-κ activity;
- administering the composition to the cell;
- exposing the cell to the insult;
- measuring the activity of the RPTP-κ in the cell after exposure to the insult;
- and
- identifying the composition as protecting RPTP-κ activity if the measured activity of the RPTP-κ in the cell after exposure to the insult is about the same as the known RPTP-κ activity.
A method for preventing photoaging by attenuating activity of epidermal growth factor receptor (EGFR) following UV irradiation of a cell, the cell expressing EGFR and RPTP-κ, comprising inhibiting the oxidation of RPTP-κ.
A composition comprising an alcohol extract of Laminaria japonica.
Dependencies are inferred from the claim text.