Lipidated glycoprotein particles and methods of use
AssigneeRamot at Tel-Aviv University Ltd.
InventorsRimona Margalit, Dan Peer
Life of this patent
Estimating the term…
Abstract
Lipidated micro- or macroparticles are prepared by covalently linking a glycoprotein, typically collagen, with at least one lipid. An amino group in the glycoprotein is joined with a primary amine in the lipid. These particles can be used to encapsulate active ingredients, such as drugs.
Claims35
Lipidated glycoprotein microparticles or macroparticles comprising the reaction product of at least one amino-containing glycoprotein with at least one lipid having a primary amino group.
A method of preparing lipidated glycoprotein macroparticles, comprising the steps of:
- (a) dissolving a glycoprotein;
- (b) providing a reaction vessel in which a lipid is disposed in a thin layer;
- (c) buffering the lipid to a basic pH;
- (d) admixing the dissolved glycoprotein in the reaction vessel;
- (e) adding a crosslinker, and optionally adding a bioactive agent stable at a basic pH;
- (f) incubating the glycoprotein and lipid and cross-linker reaction mixture, with continuous shaking, for an amount of time and under temperature conditions sufficient for lipidated glycoprotein disks to form;
- (g) buffering a resultant lipidated glycoprotein disk to a neutral pH;
- (h) separating unbound glycoprotein by centrifugation;
- and
- (i) lyophilizing the resultant lipidated glycoprotein disk to produce lipidated glycoprotein microparticles.
In a method for treating an indication with a drug that is effective for treating said indication, the improvement wherein said drug is administered encapsulated in lipidated glycoprotein microparticles or macroparticles.
35-36. (canceled)
Dependencies are inferred from the claim text.