Novel microorganism and process for treatment of organic solid matter using the microorganism

A novel microorganism belonging to genus Geobacillus, which has an ability to produce a solubilizing enzyme for solubilizing organic solid matter such as organic sludge or biological sludge, said microorganism having the following mycological characteristics:

• (a) morphological characteristics, wherein the shape and size of the microorganism is rod-shaped with a width of 0.7 to 0.8 μm and a length of 2.0 to 4.0 μm, wherein motility of the microorganism is present;
• and wherein a spore is present;
• (b) cultivating characteristics (Nutrient agar plate culture), wherein colony morphology of the microorganism is circular, entirely smooth edge and low convex;
• wherein the organism has a cream color;
• and wherein gloss is present;
• (c) physiological characteristics, wherein the microorganism is gram positive;
• wherein nitrate reduction is negative;
• wherein indole production is negative;
• wherein hydrogen sulfide production is negative;
• wherein the use of citric acid is negative;
• wherein urease is negative;
• wherein oxidase is positive;
• wherein catalase is positive;
• wherein attitude to oxygen is aerobic;
• wherein O-F test (glucose) is negative/negative;
• and wherein production of acid and gas from saccharides is D-glucose:
• acid positive/gas negative.

A novel microorganism belonging to genus Geobacillus, which has an ability to produce a solubilizing enzyme for solubilizing organic solid matter such as organic sludge or biological sludge, said microorganism having the following mycological characteristics:

• (a) morphological characteristics, wherein the shape and size of the microorganism is rod-shaped with a width of 0.7 to 0.8 μm and a length of 2.0 to 4.0 μm, wherein the motility of the microorganism is present;
• and wherein a spore is present;
• (b) cultivating characteristics (Nutrient agar plate culture), wherein colony morphology of the microorganism is circular, entirely smooth edge and low convex;
• wherein the organism has a cream color;
• and wherein gloss is present;
• (c) physiological characteristics, wherein the microorganism gram is positive;
• wherein nitrate reduction is negative;
• wherein indole production is negative;
• wherein hydrogen sulfide production is negative;
• wherein the use of citric acid is negative;
• wherein urease is negative;
• wherein oxidase is positive;
• wherein catalase is positive;
• wherein attitude to oxygen is aerobic;
• wherein O-F test (glucose) is negative/negative;
• and wherein production of acid and gas from saccharides is D-glucose:
• acid positive/gas negative;
• wherein in a fermentability test, D-glucose is positive, D-fructose is positive, D-mannose is positive, D-sorbitol is negative, inositol is negative, maltose is positive;
• and trehalose is positive;
• and wherein other physiological characteristics include β-Galactosidase activity that is negative, arginine dihydrolase activity that is negative, lysine dicarboxylase activity that is negative, tryptophan deaminase activity that is negative, acetoin production that is negative, acetoin production that is negative, gelatinase activity that is positive, and omithine dicarboxylase activity that is negative.