Methods of controlling proliferation and differentiation of stem and progenitor cells
AssigneesGamida Cell Ltd., Hadasit Medical Research Services and Development Ltd.
InventorsTony Peled, Eitan Fibach, Avi Treves
Life of this patent
Estimating the term…
Abstract
A method of ex-vivo expanding a population of stem cells, while at the same time inhibiting differentiation of the stem cells. The method comprises ex-vivo providing the stem cells with conditions for cell proliferation and with at least one copper chelator in an amount and for a time period for permitting the stem cells to proliferate and, at the same time, for reducing a capacity of the stem cells to differentiate
Claims25
An ex-vivo expanded cell population comprising cells having reduced available intracellular copper as compared to ex-vivo cells cultured in the absence of a transition metal chelator having affinity for copper and wherein said cells are further characterized by increased clonogenic potential, engraftment potential, reconstitution potential and/or proportion of undifferentiated cells.
An ex-vivo expanded cell population comprising ex-vivo expanded cells characterized by:
- (a) having been cultured for at least one week in the presence of cytokines and a transition metal chelator having affinity for copper, and
- (b) having a greater proportion of undifferentiated cells following at least 1 week culture in the presence of said transition metal chelator, as compared to ex vivo cells cultured for the same time in a presence of said cytokines yet in an absence of said transition metal chelator.
A method for ex-vivo expanding and enhancing clonogenic potential, engraftment potential, reconstitution potential and/or proportion of undifferentiated cells in a population of cells, the method comprising providing said cells with conditions for proliferation and reducing available intracellular copper of said cells as compared to ex-vivo cells cultured in the absence of a transition metal chelator having affinity for copper.
Dependencies are inferred from the claim text.