Alkaliphilic Bacillus Species alpha-Amylase Variants, Compositions Comprising alpha-Amylase Variants, And Methods of Use
OwnerBrian E. Jones, Casper Vroemen +5inventors
InventorsBrian E. Jones, Casper Vroemen, Claudine Chang, Corey Naab, Hans De Nobel, Marc Kolkman, Walter Weyler
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Abstract
Disclosed are variants of the α-amylase derived from Bacillus sp. no. 707, compositions comprising said variants, compositions comprising the variants, and methods of using the variants. The methods of use include methods of cleaning surfaces, laundering textiles, desizing, hydrolyzing biofilms off various substrates, and treating starch (e.g., liquefaction and saccharification).
Claims55
A manual or automatic dishwashing composition comprising a Bacillus sp. no. 707 α-amylase or variant thereof, and one or more of a surfactant, detergent builder, a complexing agent, a polymer, a bleach, CaCl2, a bleaching system, a stabilizer, a foam booster, a suds suppressor, an anti-corrosion agent, a soil-suspending agent, an anti-soil redeposition agent, a dye, a bactericide, a hydrotope, a tarnish inhibitor, and a perfume.
A laundry detergent additive comprising a Bacillus sp. no. 707 α-amylase or variant thereof.
An isolated nucleic acid encoding a Bacillus sp. no. 707 α-amylase or a variant thereof, wherein the variant has a residue substitution or deletion selected from the group consisting of substitutions of M202, M208, S255, R172, and/or M261 of SEQ ID NO:
- 3.
An α-amylase comprising SEQ ID NO:
- 3 or a variant thereof, wherein said variant comprises a residue substitution or deletion at residue M202, M208, and/or M261 of SEQ ID NO:3.
A method of hydrolyzing a biofilm comprising administering the composition of any of claims 44 or 45 for a period sufficient to process said biofilm.
Dependencies are inferred from the claim text.