Repeatable Protein Arrays
AssigneeThe Babraham Institute
InventorsMichael J. Taussig, Mingyue He
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Abstract
The invention relates to a method of producing a protein array (5) on one support surface (3) from a corresponding nucleic acid array on a separate surface (1), to protein arrays produced by the method, to uses of the protein arrays in the identification of interactions between arrayed proteins and other molecules, and to kits for producing said protein arrays.
Claims32
1-37. (canceled)
A method of producing a protein array comprising:
- a) exposing a nucleic acid array on a first support surface to a cell-free system wherein the cell-free system is capable of performing protein synthesis by transcription and translation;
- b) expressing a plurality of proteins from said nucleic acid array;
- and
- c) immobilizing said proteins as a corresponding array on a second support surface, characterised in that said second support surface is directly or indirectly in contact with said first support surface.
A method of producing a protein array comprising:
- a) immobilising protein-encoding DNA molecules capable of being transcribed and translated by a cell-free system on a first support surface;
- b) placing a protein permeable material carrying a cell-free system capable of performing protein synthesis by transcription and translation between said first support surface and a second support surface carrying a protein immobilisation agent;
- c) expressing a plurality of proteins from said DNA molecules;
- and
- d) immobilizing said plurality of proteins on said second protein immobilising support surface as they are expressed so as to generate a corresponding protein array.
A method of producing a protein array comprising:
- a) immobilising protein-encoding DNA molecules, capable of being transcribed and translated by a cell-free system, on a first support surface;
- b) applying a cell-free system, capable of performing protein synthesis by transcription and translation, to said first support surface;
- c) placing said first support surface in contact with a second support surface carrying a protein immobilisation agent;
- d) expressing a plurality of proteins from said DNA molecules;
- and
- e) immobilising said plurality of proteins on said second protein immobilising support surface as they are expressed so as to generate a corresponding protein array.
A protein array comprising:
- a second support surface having a plurality of proteins immobilized thereto, wherein the proteins are produced by cell-free protein synthesis from a corresponding nucleic acid array template located on a separate first support surface, characterised in that said second support surface is directly or indirectly in contact with said first support surface such that the proteins, when translated, are immobilized on the second support surface.
A method of identifying interactions of a plurality of proteins on a protein array comprising:
- a) providing a plurality of proteins arranged on a protein array produced on a second support surface by cell-free protein synthesis from a corresponding nucleic acid array template located on a separate first support surface, characterised in that said second support surface is directly or indirectly in contact with said first support surface;
- and
- b) identifying interactions of the proteins on the protein array with one or more molecules exposed to the second support surface.
A kit for producing a protein array comprising:
- (i) a first support surface having protein-encoding DNA molecules immobilised thereon;
- and
- (ii) a second support surface having a protein immobilisation agent immobilised thereon, wherein said first and second support surfaces allow direct or indirect contact between said first and second support surfaces.
Dependencies are inferred from the claim text.