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US 2008/0293585 A1Published application
5'/3' Ratioing Procedure for Detection of Gene Rearrangements
AssigneeCedars-Sinai Medical Center
InventorsGabor Szabo, Gyorgy Lustyik
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Abstract
The present invention relates to methods and kits useful for the detection of gene rearrangements and the diagnosis of a propensity to develop a disease condition caused by the gene rearrangements, wherein two PCR products are prepared from the 5′ side and from the 3′ side of a putative breakpoint of the gene of interest, and the ratio of the two products are measured.
Claims40
A method of detecting a gene rearrangement, comprising:
- providing a quantity of a polynucleotide, said polynucleotide including a region of interest;
- cleaving said polynucleotide to create polynucleotide fragments, a portion of which are capable of binding to a probe;
- combining a quantity of the probe with said polynucleotide fragments to hybridize at least a portion of the polynucleotide fragments;
- removing a substantial quantity of the hybridized polynucleotide fragments to create a remaining sample and a removed sample;
- and
- quantitating the amount of a first section of the region of interest and a second section of the region of interest in the remaining sample or the removed sample to detect the gene rearrangement.
3 more elements
A method to diagnose a propensity to develop a disease condition, comprising:
- providing a quantity of a polynucleotide, said polynucleotide including a region of interest;
- cleaving said polynucleotide to create polynucleotide fragments, a portion of which are capable of binding to a probe;
- combining a quantity of the probe with said polynucleotide fragments to hybridize at least a portion of the polynucleotide fragments;
- removing a substantial quantity of the hybridized polynucleotide fragments to create a remaining sample and a removed sample;
- quantitating the amount of a first section of the region of interest and a second section of the region of interest in the remaining sample or the removed sample to detect the gene rearrangement and diagnose the propensity to develop a disease condition.
2 more elements
A kit for detecting a gene rearrangement, comprising:
- a restriction enzyme;
- a probe;
- and
- instructions to use the restriction enzyme and the probe to detect the gene rearrangement.
1 more element
Dependencies are inferred from the claim text.