Detection of Carbohydrate Biomarkers
AssigneeThe Curators of the University of Missouri
InventorsSusan L. Deutscher, Thomas P. Quinn, Edward R. Sauter
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Abstract
The present invention generally relates to detection of carbohydrate biomarkers in nipple aspirate fluid samples. One aspect of the invention is a method for assaying a nipple aspirate fluid for the presence of TF or Tn carbohydrate biomarker. The assay generally employs an immobilized capture agent specific for TF or Tn and can be further coupled to either direct or indirect detection of bound TF or Tn carbohydrate biomarker through the use of a labeled binding agent.
Claims39
A method for detection of biomarkers comprising assaying nipple aspirate fluid (NAF) derived from a subject for the presence of a carbohydrate biomarker occurring on protein, lipid or carbohydrate molecules dissolved in the NAF, said carbohydrate biomarker being selected from the group consisting of TF, Tn, and both TF and Tn.
A method for detection of biomarkers comprising assaying nipple aspirate fluid (NAF) or a dilution or concentrate thereof derived from a subject for the presence of a secreted carbohydrate biomarker, said carbohydrate marker being selected from the group consisting of TF, Tn, and both TF and Tn, said method comprising the steps of:
- combining a NAF or NAF dilution or concentrate sample with a capture agent bound to a carrier, wherein the capture agent binds specifically to the secreted carbohydrate biomarker when present in the NAF or NAF dilution or concentrate sample;
- and
- detecting the carbohydrate biomarker bound to the capture agent.
A method for detection of biomarkers comprising assaying nipple aspirate fluid (NAF) for a carbohydrate biomarker displayed by a secreted protein or lipid in the NAF, said carbohydrate biomarker being selected from the group consisting of TF, Tn, and both TF and Tn.
Dependencies are inferred from the claim text.