Method of Detecting Structural Change in Target Sugar Chain
OwnerMasayuki Machida, Jun Natsuki +2inventors
InventorsMasayuki Machida, Jun Natsuki, Koichi Tamano, Hideji Tajima
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Abstract
A method of easily and efficiently detecting any structural change in a target sugar chain that can be present as a first or a second sugar chain depending on the structural change. In the method, the target sugar chain is mixed with a first to an nth lectin that can bind to the first and second sugar chains and the ratio between the amounts of the first and second lectins as bound to the target sugar chain is used as an index for detecting the presence or absence of a structural change in the target sugar chain.
Claims8
A method for detecting the presence or absence of a structural change in a target sugar chain, in which the target sugar chain which can be present as a first or second sugar chain depending on the structural change is mixed with a first to an nth lectin (n is an integer of 2 or more) that can bind to the first and second sugar chains, and the ratio between the amount of the first lectin binding to the sugar chain and the amount of the second lectin binding to the sugar chain is used as an index,
- the first to the nth lectin satisfying the following relation (I):
- B
- 11
- /
- B
- 12
- B
- 21
- /
- B
- 22
- >
- A
- 11
- /
- A
- 12
- A
- 21
- /
- A
- 22
- >
- 1
- Relation
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-
- 1
- [wherein, A11 represents the amount of the first lectin binding to one molecule of the first sugar chain when the first sugar chain and the first lectin are mixed in the absence of another lectin;
- A12 represents the amount of the second lectin binding to one molecule of the first sugar chain when the first sugar chain and the second lectin are mixed in the absence of another lectin;
- A21 represents the amount of the first lectin binding to one molecule of the second sugar chain when the second sugar chain and the first lectin are mixed in the absence of another lectin;
- A22 represents the amount of the second lectin binding to one molecule of the second sugar chain when the second sugar chain and the second lectin are mixed in the absence of another lectin;
- B11 represents the amount of the first lectin binding to one molecule of the first sugar chain when the first sugar chain is mixed with the first to the nth lectin;
- B12 represents the amount of the second lectin binding to one molecule of the first sugar chain when the first sugar chain is mixed with the first to the nth lectin;
- B21 represents the amount of the first lectin binding to one molecule of the second sugar chain when the second sugar chain is mixed with the first to the nth lectin;
- and B22 represents the amount of the second lectin binding to one molecule of the second sugar chain when the second sugar chain is mixed with the first to the nth lectin.]
Dependencies are inferred from the claim text.