Cell for Producing Retrovirus Vector
AssigneeTakara Bio Inc.
InventorsHideto Chono, Hiromi Okuyama, Tomoe Egashira, Nobuto Koyama, Junichi Mineno, Ikunoshin Kato
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Abstract
The N-acetylglucosaminyltransferase III activity is enhanced in a cell carrying retrovirus-origin gag-pol gene and env gene. By constructing a retrovirus vector with the use of the above cell, a retrovirus vector having a modified sugar chain structure can be obtained. The retrovirus vector constructed by this method shows a high infection efficiency particularly in the presence of a functional substance.
Claims13
A cell having a gag-pol gene and an env gene derived from a retrovirus, in which an N-acetylglucosaminyltransferase III activity is enhanced.
A retrovirus producer cell, which has a gag-pol gene and an env gene derived from a retrovirus, in which an N-acetylglucosaminyltransferase III activity is enhanced, and which is transformed with a recombinant retrovirus vector or a recombinant retrovirus vector plasmid.
A method for producing a retrovirus vector, the method comprising:
- culturing a retrovirus producer cell, which has a gag-pol gene and an env gene derived from a retrovirus, in which an N-acetylglucosaminyltransferase III activity is enhanced, and which is transformed with a recombinant retrovirus vector or a recombinant retrovirus vector plasmid;
- and
- collecting a culture supernatant.
A retrovirus vector, which is produced according to a method for producing a retrovirus vector, wherein the method comprises:
- culturing a retrovirus producer cell, which has a gag-pol gene and an env gene derived from a retrovirus, in which an N-acetylglucosaminyltransferase III activity is enhanced, and which is transformed with a recombinant retrovirus vector or a recombinant retrovirus vector plasmid;
- and
- collecting a culture supernatant.
A method for transferring a gene into a target cell, the method comprising:
- culturing a retrovirus producer cell, which has a gag-pol gene and an env gene derived from a retrovirus, in which an N-acetylglucosaminyltransferase III activity is enhanced, and which is transformed with a recombinant retrovirus vector or a recombinant retrovirus vector plasmid;
- collecting a culture supernatant to obtain a retrovirus vector;
- and
- infecting a target cell with the retrovirus vector in the presence of a functional substance having a retrovirus-binding activity.
Dependencies are inferred from the claim text.