Sensor of pyrophosphate and snp typing sensor using the same

A SNP typing sensor comprising

• an insulative substrate,
• an electrode system that is formed on the insulative substrate and has at least a measurement electrode and a counter electrode,
• a measuring cavity provided on the substrate so as to surround the electrode system,
• a reaction cavity provided on the substrate, and
• a flow path that connects the measuring cavity with the reaction cavity, wherein:
• pyrophosphatase, glyceraldehyde-3-phosphate dehydrogenase, diaphorase, glyceraldehyde-3-phosphate, a nucleotide, an electronic mediator, and a buffer component are carried in the measuring cavity;
• the pyrophosphatase is separated from the buffer component;
• the glyceraldehyde-3-phosphate dehydrogenase is separated from the buffer component;
• the glyceraldehyde-3-phosphate is separated from every one of the buffer component, the pyrophosphatase, and the glyceraldehyde-3-phosphate dehydrogenase;
• and
• the nucleotide is oxidized nicotineamide adenine dinucleotide, oxidized nicotineamide adenine dinucleotide phosphate, or a combination thereof.