Multimeric Oxidoreductases
AssigneeGenencor International, Inc.
InventorsRobert M. Caldwell, M. Harunur Rashid, Fernando Valle
Life of this patent
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Abstract
The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof.
Claims9
1-6. (canceled)
An isolated polynucleotide coding for a multimeric oxidoreductase complex which functions in the enzymatic conversion of a carbon substrate to a desired product, said complex having an amino acid sequence comprising an alpha subunit, a gamma subunit and a cytochrome C subunit, wherein
- the alpha subunit has dehydrogenase activity and is selected from the sequences
- of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, SEQ ID NO:
- 13, SEQ ID NO:14, SEQ ID NO:
- 15, SEQ ID NO:
- 16, SEQ ID NO:
- 17, SEQ ID NO:
- 18 and SEQ ID NO:
- 19 and sequences having at least 95% sequence identity thereto;
- the gamma subunit is selected from the sequences of SEQ ID NO:
- 39, SEQ ID NO:
- 40, SEQ ID NO:
- 41, SEQ ID NO:
- 42, SEQ ID NO:
- 43, SEQ ID NO:
- 44, SEQ ID NO:
- 45, SEQ ID NO:
- 46, SEQ ID NO:
- 47, SEQ ID NO:
- 48, SEQ ID NO:
- 49, SEQ ID NO:
- 50, SEQ ID NO:
- 51, SEQ ID NO:
- 52, SEQ ID NO:
- 53, SEQ ID NO:
- 54, SEQ ID NO:
- 55, SEQ ID NO:
- 56, SEQ ID NO:
- 57 and sequences having at least 95% sequence identity thereto;
- and
- the cytochrome C subunit is selected from the group of sequences of SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 22, SEQ ID NO:
- 23, SEQ ID NO:
- 24, SEQ ID NO:
- 25, SEQ ID NO:
- 26, SEQ ID NO:
- 27, SEQ ID NO:
- 28, SEQ ID NO:
- 29, SEQ ID NO:
- 30, SEQ ID NO:
- 31, SEQ ID NO:
- 32, SEQ ID NO:
- 33, SEQ ID NO:
- 34, SEQ ID NO:
- 35, SEQ ID NO:
- 36, SEQ ID NO:
- 37, SEQ ID NO:
- 38 and sequences having at least 95% sequence identity thereto.
8-37. (canceled)
Dependencies are inferred from the claim text.