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US 2008/0293123 A1Published application
Compositions comprising viruses and methods for concentrating virus preparations
AssigneeSchering Corporation
InventorsAndreas Frei, Henry K.H. Kwan, Varda E. Sandweiss, Gary J. Vellekamp, Pui-Ho Yuen, Laureano L. Bondoc, JR., Frederick William Porter, IV, John Chu-Tay Tang, Peter Ihnat
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Abstract
A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.
Claims5
1-28. (canceled)
A purified recombinant adenovirus composition obtained by a method of purifying a virus preparation, the method comprising the steps of:
- a) subjecting the virus preparation to anion-exchange chromatography, wherein the virus is eluted as a virus preparation product from an anion-exchange chromatographic medium;
- b) adding a polyhydroxy hydrocarbon to the virus preparation product of step (a) so that the concentration of polyhydroxy hydrocarbon in the product reaches a level of about 25% or more;
- and
- c) increasing the concentration of virus in the product of step (b) by applying pressure to the product such that diluent is removed from the product through a filter while the virus is retained,
- wherein the composition has a virus concentration of about 1×109 to 1×1013 particles/ml.
2 more elements
Dependencies are inferred from the claim text.