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US 2008/0293120 A1Published application
Lipolytic enzymes variants
AssigneeNovozymes A/S
InventorsKim Borch, Luise Erlandsen, Jesper Vind, Allan Svendsen, Christel T. Jorgensen
Life of this patent
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Abstract
The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
Claims15
1-14. (canceled)
A method of producing a polypeptide, comprising:
- (a) selecting an amino acid sequence for a fungal lipolytic enzyme,
- (b) selecting an amino acid residue in the sequence which corresponds to A29, K33, I83 or A255 of SEQ ID NO:
- 1,
- (c) modifying the amino acid sequence by substituting or deleting the selected residue,
- (d) preparing a polypeptide having the modified amino acid sequence, and
- (e) adding the polypeptide to a dough and testing dough stickiness.
3 more elements
A polypeptide which:
- (a) has hydrolytic activity towards an ester bond in a polar lipid, and
- (b) has an amino acid sequence which
- (i) has at least 80% identity to SEQ ID NO:
- 1 and has a different amino acid or an amino acid deletion at a position corresponding to A29, K33, I83 or A255, or
- (ii) has at least 80% identity to SEQ ID NO:
- 2 and has a different amino acid or an amino acid deletion at a position corresponding to R84 or P256.
3 more elements
Dependencies are inferred from the claim text.