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US 2008/0293107 A1Published application
Methods for using riboprimers for strand displacement replication of target sequences
AssigneeEpicentre Technologies Corporation
InventorsGary A. Dahl, Jerome J. Jendrisak, Agnes J. Radek
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Abstract
Methods, compositions and kits for amplifying a target sequence by strand displacement replication using strand-displacing primers. The method uses primers that have only ribonucleotides or purine ribonucleotides and at least one 2′-substituted pyrimidine-2′-deoxyribonucleotide.
Claims20
A method for amplifying a target nucleic acid sequence comprising a target nucleic acid:
- a) hybridizing a Riboprimer to a single stranded DNA template comprising the target nucleic acid sequence;
- b) optionally hybridizing a blocking oligo to a region of the template which is 5′ with respect to hybridization of the Riboprimer to the template;
- c) extending the Riboprimer with a DNA polymerase;
- and
- d) cleaving the annealed Riboprimer with an RNAse H enzyme such that another Riboprimer hybridizes to the template and repeats primer extension by strand displacement, whereby multiple copies of the complementary sequence of the target sequence are produced.
2 more elements
Dependencies are inferred from the claim text.