Method for the Fermentative Production of L-Amino Acids With the Aid of Coryneform Bacteria Capable of Using Glycerin as the Only Carbon Source

3:

• The coryneform bacterium as claimed in claim 2, wherein the polypeptides have amino acid sequences which are at least 95% identical with one of the sequences selected from group a) to r).

4:

• The coryneform bacterium as claimed in claim 2, wherein the polypeptides have amino acid sequences which are 100% identical with one of the sequences selected from group a) to r).

6:

• The coryneform bacterium as claimed in claim 1, wherein said bacterium is produced by transformation, conjugation or a combination of these methods using a vector which contains at least one or more of said polynucleotides or alleles thereof and if appropriate a promoter.

7:

• The coryneform bacterium as claimed in claim 1, in which the heterologous expression is effected by integration of the gene or of the alleles into the chromosome of the bacteria.

8:

• The coryneform bacterium as claimed in claim 1, wherein the heterologous expression is achieved by an extrachromosomally replicating vector.

9:

• The coryneform bacterium as claimed in claim 1, wherein the expression of at least one of said polynucleotides is under the control of a promoter amplifying the expression of the gene.

10:

• The coryneform bacterium as claimed in claim 1, wherein the coryneform bacterium is a bacterium selected from the group Corynebacterium efficiens, Corynebacterium glutamicum, Corynebacterium thermoaminogenes, and Corynebacterium aminoagenes.

11:

• The coryneform bacterium as claimed in claim 1, wherein said bacterium is Corynebacterium glutamicum.

12:

• The coryneform bacterium as claimed in claim 1, wherein said bacterium is an L-amino acid-excreting bacterium.

13:

• The coryneform bacterium as claimed in claim 1, wherein said bacterium is an L-lysine- or L-tryptophan-excreting bacterium.

14:

• The coryneform bacterium as claimed in claim 13, wherein, in addition, further genes of the biosynthesis pathway of the desired L-amino acid are present in an amplified form.

15:

• The coryneform bacterium as claimed in claim 1, in which the heterologous polynucleotide expressed originates from a prokaryotic organism.

16:

• The coryneform bacterium as claimed in claim 1, in which the heterologous polynucleotide expressed originates from Enterobacteriaceae.

17:

• The coryneform bacterium as claimed in claim 1, in which the heterologous polynucleotide expressed originates from Escherichia coli.

18:

• A recombinant coryneform bacterium in which at least the endogenous polynucleotides of glycerol metabolism glpD and glpK are overexpressed.

19:

• A method for the fermentative production of L-amino acids comprising:
• a) culturing the recombinant coryneform bacteria producing the desired L-amino acid as claimed in claim 1 in a medium containing glycerol or, if appropriate, in addition one or more further carbon sources under conditions in which the desired L-amino acid accumulates in the medium or in the cells, and, if appropriate,
• b) isolating the desired L-amino acid, in which if appropriate components of the fermentation broth and/or biomass remain in the end product in their totality or in fractions (>0 to 100%).

20:

• The method as claimed in claim 19, wherein, as carbon source, ≧10 to 100% glycerol is used.

21:

• The method as claimed in claim 19, wherein L-lysine or L-tryptophan is produced.

22:

• The method as claimed in claim 19, wherein bacteria in which the metabolic pathways which increase the formation of the desired L-amino acid have been at least in part amplified.

23:

• The method as claimed in claim 19, wherein bacteria in which the metabolic pathways which decrease the formation of the desired L-amino acid have been at least in part switched off.

24:

• The method as claimed in claim 22, wherein, for the production of L-amino acids, coryneform bacteria are fermented in which at the same time one or more of the genes selected from the group accBC, accDA, cstA, cysD, cysE, cysH, cysK, cysN, cysQ, dapA, dapB, dapC, dapD, dapE, dapF, ddh, dps, eno, gap, gap2, gdh, gnd, lysC, lysCFBR, lysE, msiK, opcA, oxyR, ppc, ppcFBR, pgk, pknA, pknB, pknD, pknG, ppsA, ptsH, ptsI, ptsM, pyc, pyc P458S, sigC, sigD, sigE, sigH, sigM, tal, thyA, tkt, tpi, zwa1, zwf and zwf A243T is or are amplified.

25:

• The method as claimed in claim 22, wherein the activity or concentration of the protein(s) which is (are) encoded by the amplified gene(s) increases by in each case 10 to 2000%, based on that of the wild type protein or the activity or concentration of the protein in the starting microorganism.

26:

• The method as claimed in claim 23, wherein, for production of L-amino acids, corynefomm bacteria are fermented in which at the same time one or more of the genes selected from the group aecD, ccpA1, ccpA2, citA, citB, citE, fda, gluA, gluB, gluC, gluD, luxR, luxS, lysR1, lysR2, lysR3, menE, mqo, pck, pgi, poxB and zwa2 is or are attenuated.

27:

• The method as claimed in claim 26, wherein the activity or concentration of the protein(s) which is (are) encoded by the attenuated gene(s) falls in each case to 0 to 75%, based on that of the wild type protein or the activity or concentration of the protein in the starting microorganism.