Tumor mouse models using lentiviral vectors
AssigneeThe Salk Institute for Biological Studies
InventorsTomotoshi Marumoto, Inder Verma, Yasushi Soda, Yifeng Xia
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Abstract
This invention provides model systems for study of tumors. Model cells and model animals are prepared to mimic characteristics of natural clinical tumors. The tumor models can be contacted with putative modulators to screen for therapeutics and to help understand tumorigenic processes. Model animals can include model cells, closely correlated to the characteristics of clinical tumors, in an environment closely mimicking that of the tumors in patients. Thus, the animal models are highly representative for use in clinical and pharmacological studies.
Claims24
A method of generating localized foci of transduced cells in vivo, the method comprising:
- providing a vector system comprising one or more recombinant nucleic acid comprising an encoded sequence of interest;
- providing an animal;
- injecting the vector system into an organ of the animal at a desired location;
- and,
- transducing the recombinant nucleic acid from the vector system into target cells at the desired location;
- thereby generating a localized foci of transduced target cells in the organ.
A composition comprising a recombinant nucleic acid that comprises:
- a sequence comprising a first lox recombination site and a second lox recombination site bracketing a nucleic acid sequence encoding a stuffer sequence;
- a promoter sequence upstream from the lox recombination sites;
- a coding sequence of interest and a first marker nucleic acid sequence encoding a first marker, each downstream from the lox recombination sites;
- and,
- a lentiviral packaging site (psi).
A non-human recombinant laboratory animal, comprising:
- a recombinant Cre recombinase, expressed in one or more cell or tissue of the animal;
- and,
- a recombinant nucleic acid that encodes a sequence comprising:
- i) a first lox recombination site and a second lox recombination site bracketing a nucleic acid sequence encoding a stuffer sequence, or comprising a recombinant nucleic acid that encodes a sequence comprising recombined lox recombination sites;
- ii) a promoter sequence upstream from the recombined lox recombination sites or upstream from the first and second lox sites;
- iii) a coding sequence of interest and a first marker nucleic acid sequence encoding a first marker, each downstream from the recombined lox recombination sites or downstream from the first and second lox sites;
- and,
- iv) a lentiviral packaging site (psi).
Dependencies are inferred from the claim text.