Method for identifying hair conditioner-resistant hair-binding peptides and hair benefit agents therefrom

A hair conditioner-resistant hair-binding peptide identified by a process comprising the steps of:

• a) providing a combinatorial library of DNA associated peptides;
• b) contacting the library of (a) with a hair sample wherein the hair complexes with the DNA associated peptides to form a reaction solution comprising DNA associated peptide-hair complexes;
• c) isolating the DNA associated peptide-hair complexes of (b) from the reaction solution;
• d) contacting the isolated DNA associated peptide-hair complexes of (c) with a hair conditioner matrix to form a conditioning solution wherein the concentration of the hair conditioner matrix is at least about 10% of full strength concentration;
• e) isolating the DNA associated peptide-hair complexes of (d) from the conditioning solution;
• f) amplifying the DNA encoding the peptide portion of the DNA associated peptide-hair complexes of (e);
• and
• g) sequencing the amplified DNA of (f) encoding a conditioner resistant hair-binding peptide wherein the conditioner-resistant hair-binding peptide is identified.

A diblock, peptide-based hair benefit agent having the general structure (HCPm)n—BA, wherein;

• a) HCP is a hair conditioner-resistant hair-binding peptide;
• b) BA is a benefit agent;
• c) m ranges from 1 to about 100;
• and
• d) n ranges from 1 to about 50,000.

A triblock, peptide-based hair benefit agent having the general structure [(HCPX—S)m]n—BA, wherein;

• a) HCP is a hair conditioner-resistant hair-binding peptide;
• b) BA is a benefit agent;
• c) S is a spacer;
• d) x ranges from 1 to about 10;
• e) m ranges from 1 to about 100;
• and
• f) n ranges from 1 to about 50,000.

A method for coloring hair, eyebrows or eyelashes comprising the steps of:

• a) providing a hair coloring composition comprising a hair colorant selected from the group consisting of:
• i) (HCPm)n—C;
• and
• ii) [(HCPX—S)m]n—C
• wherein:
• 1) HCP is a hair conditioner-resistant hair-binding peptide;
• 2) C is a coloring agent;
• 3) n ranges from 1 to about 50,000;
• 4) S is a spacer;
• 5) m ranges from 1 to about 100;
• and
• 6) x ranges from 1 to about 10;
• and wherein the hair conditioner-resistant hair-binding peptide is selected by a method comprising the steps of:
• A) providing a combinatorial library of DNA associated peptides;
• B) contacting the library of (A) with a hair sample wherein the hair complexes with the DNA associated peptides to form a reaction solution comprising DNA associated peptide-hair complexes;
• C) isolating the DNA associated peptide-hair complexes of (B) from the reaction solution;
• D) contacting the isolated DNA associated peptide-hair complexes of (C) with a hair conditioner matrix to form a conditioning solution wherein the concentration of the hair conditioner matrix is at least about 10% of full strength concentration;
• E) isolating the DNA associated peptide-hair complexes of (D) from the conditioning solution;
• F) amplifying the DNA encoding the peptide portion of the DNA associated peptide-hair complexes of (E);
• and
• G) sequencing the amplified DNA of (F) encoding a hair conditioner resistant hair-binding peptide wherein the hair conditioner-resistant hair-binding peptide is identified;
• and
• b) applying the hair coloring composition of (a) to hair, eyebrows or eyelashes for a time sufficient for the hair colorant to bind to hair, eyebrows or eyelashes.

A method for forming a protective layer of a peptide-based conditioner on hair comprising the steps of:

• a) providing a hair care composition comprising a hair conditioner selected from the group consisting of:
• i) (HCPm)n—HCA;
• and
• ii) [(HCPX—S)m]n—HCA
• wherein:
• 1) HCP is a hair conditioner-resistant hair-binding peptide;
• 2) HCA is a hair conditioning agent;
• 3) n ranges from 1 to about 50,000;
• 4) S is a spacer;
• 5) m ranges from 1 to about 100;
• and
• 6) x ranges from 1 to about 10;
• and wherein the hair conditioner-resistant hair-binding peptide is selected by a method comprising the steps of:
• A) providing a combinatorial library of DNA associated peptides;
• B) contacting the library of (A) with a hair sample wherein the hair complexes with the DNA associated peptides to form a reaction solution comprising DNA associated peptide-hair complexes;
• C) isolating the DNA associated peptide-hair complexes of (B) from the reaction solution;
• D) contacting the isolated DNA associated peptide-hair complexes of (C) with a hair conditioner matrix to form a conditioning solution wherein the concentration of the hair conditioner matrix is at least about 10% of full strength concentration;
• E) isolating the DNA associated peptide-hair complexes of (D) from the conditioning solution;
• F) amplifying the DNA encoding the peptide portion of the DNA associated peptide-hair complexes of (E);
• and
• G) sequencing the amplified DNA of (F) encoding a conditioner resistant hair-binding peptide wherein the conditioner-resistant hair-binding peptide is identified;
• and
• b) applying the hair care composition of (a) to hair and allowing the formation of said protective layer.