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US 2008/0290283 A1Published application
Signal noise reduction for imaging in biological analysis
AssigneeApplera Corporation
InventorsAustin B. Tomaney, Mark F. Oldham
Life of this patent
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Abstract
A system and method for characterizing contributions to signal noise associated with charge-coupled devices adapted for use in biological analysis. Dark current contribution, readout offset contribution, photo response non-uniformity, and spurious charge contribution can be determined by the methods of the present teachings and used for signal correction by systems of the present teachings.
Claims19
A method comprising:
- providing a detector comprising a plurality of pixels that are capable of forming an optical image of fluorescent light emitted from a biological sample;
- using the detector to detect a first fluorescent signal from a biological sample;
- generating a first electronic signal that corresponds to said fluorescent signal;
- characterizing a signal noise component of the first electronic signal as a characterized signal noise component comprising a dark current contribution;
- manipulating the detector to reduce signal noise based upon the signal noise component, and to form a manipulated detector;
- using the manipulated detector to detect a second fluorescent signal from the biological sample;
- and
- using the manipulated detector to generate a second electronic signal.
5 more elements
A method comprising:
- providing a sequence analysis system, the sequence analysis system comprising a detector and a charge-coupled device, the detector comprising a plurality of pixels that are capable of forming an optical image of fluorescent light emitted from a biological sample, and the readout component being capable of reading an output signal from each pixel, wherein each output signal comprises a charge collected and transferred from a respective pixel;
- using the detector to detect a first fluorescent signal from a biological sample;
- generating a first electronic signal that corresponds to the first fluorescent signal, and which comprises a charge collected and transferred from each pixel;
- using the readout component to generate a readout of the charge collected and transferred from each pixel;
- characterizing a signal noise component of the first electronic signal as a characterized signal noise component comprising a readout offset contribution;
- manipulating the detector to reduce signal noise based upon the signal noise component, and to form a manipulated detector;
- using the manipulated detector to detect a second fluorescent signal from the biological sample;
- and
- using the manipulated detector to generate a second electronic signal.
6 more elements
An imaging system for analysis of a biological sample, comprising:
- a detector comprising a plurality of pixels;
- a readout component configured to read an output signal from each of the plurality of pixels;
- a controller configured to correct signal noise from the output signal, wherein the signal noise comprises a dark current component;
- and
- a processor programmed to (i) determine an average value of the dark current component from each pixel, (ii) determine a threshold value of the dark current component, above which a pixel is identified to be a hot pixel, (iii) compare the dark current component of each pixel to the threshold value, and (iv) identify at least one hot pixel.
2 more elements
An imaging system for analysis of a biological sample, comprising:
- a detector comprising a plurality of pixels;
- a readout component configured to read an output signal from each of the plurality of pixels;
- a controller configured to induce a substantially uniform illumination condition to the detector and provide an exposure from each of the plurality of pixels for a selected exposure duration;
- and
- a processor programmed to (i) remove signal noise from the output signal of each of the plurality of pixels to yield a corrected signal for each pixel, (ii) determine an average value of the corrected signals, (iii) determine a threshold value below which a pixel is identified to be a dead pixel, (iv) compare the corrected signal of each pixel to the threshold value, and (v) identify at least one dead pixel.
2 more elements
An imaging system for analysis of a biological sample, comprising:
- a detector comprising a plurality of pixels;
- a readout component configured to read an output signal from each of the plurality of pixels;
- a controller configured to induce a substantially uniform illumination condition to the detector and provide an exposure from each of the plurality of pixels, for a selected exposure duration;
- and
- a processor programmed to (i) remove signal noise from the output signal of each pixel to yield a corrected signal for each pixel, (ii) identify at least one hot pixel and at least one dead pixel, (iii) determine an average value of the corrected signals from pixels that are not identified as hot pixels or dead pixels, (iv) determine a respective normalization factor for each of the plurality of pixels, and (v) save the normalization factor for each pixel.
2 more elements
Dependencies are inferred from the claim text.