Ex vivo methods for testing organ system disruption
AssigneeLifeline Scientific, Inc.
InventorsChristopher Gerald Curtis, John Brassil, David Kravitz
Life of this patent
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Abstract
Properties such as toxicity of substances may be determined by assaying properties, fates and effects of substances in an ex vivo metabolically active human organ or tissue under normothermic perfusion with a fluid containing a test substance. The data can be used as, for example, part of a submission to a government regulatory organization. Preferred methods use perfused endocrine gland organs or tissues to evaluate hormone or other bodily chemical disruption caused by substances and pre-donation diseased or injured organs or tissues.
Claims31
A method of testing the toxicity of a substance, comprising:
- passing a perfusate comprising the substance under normothermic conditions through at least one metabolically active ex vivo donated human organ or tissue that was injured or diseased before donation;
- collecting data derived from the perfused organ or tissue;
- and
- analyzing the data to assess toxicity of the substance.
A method of generating revenue, comprising:
- charging a fee to a third party for collecting data relating to the toxicity of a test substance;
- passing a perfusate comprising the substance under normothermic conditions through at least one metabolically active ex vivo donated human organ or tissue that was injured or diseased before donation;
- collecting data derived from the perfused organ or tissue;
- and
- providing information relative to the data to said third party.
A method of testing the toxicity of a substance, comprising:
- passing a perfusate comprising the substance under normothermic conditions through at least one metabolically active ex vivo human endocrine gland or endocrine gland tissue under normothermic conditions;
- collecting data derived from the perfused organ or tissue;
- and
- analyzing the data to assess effects of the substance on hormone uptake or production by said organ or tissue.
A method of generating revenue, comprising:
- charging a fee to a third party for collecting data relating to the toxicity of a test substance;
- passing a perfusate comprising the substance under normothermic conditions through at least one metabolically active ex vivo human endocrine gland or endocrine gland tissue under normothermic conditions;
- collecting data derived from the perfused organ or tissue relating to hormone uptake or production;
- and
- providing information relative to the data to said third party.
Dependencies are inferred from the claim text.