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US 2008/0287364 A1Published application
Bitter Taste Receptors
AssigneeDeutsches Institut für Ernährungsorschung, Stiftung des öffentlichen Rechts, Vertreten durch den Stiftungsvorstand
InventorsBernd Bufe, Thomas Hofmann, Dietmar Krautwurst, Christina Kuhn, Wolfgang Meyerhof
Life of this patent
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Abstract
The present invention relates to bitter-taste receptors and their role in bitter taste transduction. The invention also relates to assays for screening molecules that modulate, e.g. suppress or block, bitter taste transduction, or enhance bitter taste response.
Claims25
A polynucleotide selected from the group consisting of:
- (a) polynucleotides encoding at least the mature form of the polypeptide having the deduced amino acid sequence as shown in SEQ ID NO:
- 12;
- (b) polynucleotides having the coding sequence, as shown in SEQ ID NO:
- 11 encoding at least the mature form of the polypeptide;
- (c) polynucleotides encoding a fragment or derivative of a polypeptide encoded by a polynucleotide of any one of (a) to (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to said polypeptide, and said fragment or derivative has bitter substance binding activity;
- (d) polynucleotides which are at least 50% identical to a polynucleotide as defined in any one of (a) to (c) and which code for a polypeptide having bitter substance binding activity;
- and
- (e) polynucleotides the complementary strand of which hybridizes under stringent conditions to a polynucleotide as defined in any one of (a) to (d) and which code for a polypeptide having bitter substance binding activity;
- or the complementary strand of such a polynucleotide.
6 more elements
A polypeptide having the amino acid sequence encoded by a polynucleotide selected from the group consisting of:
- (a) polynucleotides encoding at least the mature form of the polypeptide having the deduced amino acid sequence as shown in SEQ ID NO:
- 12;
- (b) polynucleotides having the coding sequence, as shown in SEQ ID NO:
- 11 encoding at least the mature form of the polypeptide;
- (c) polynucleotides encoding a fragment or derivative of a polypeptide encoded by a polynucleotide of any one of (a) to (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to said polypeptide, and said fragment or derivative has bitter substance binding activity;
- (d) polynucleotides which are at least 50% identical to a polynucleotide as defined in any one of (a) to (c) and which code for a polypeptide having bitter substance binding activity;
- and
- (e) polynucleotides the complementary strand of which hybridizes under stringent conditions to a polynucleotide as defined in any one of (a) to (d) and which code for a polypeptide having bitter substance binding activity;
- or the complementary strand of such a polynucleotide;
- wherein said polypeptide is obtainable by culturing a host cell genetically engineered to comprise a polynucleotide as defined in any of (a) to (e), and recovering the polypeptide encoded by said polynucleotide.
7 more elements
A process for isolating a compound that binds to a polypeptide encoded by a polynucleotide selected from the group consisting of:
- (a) polynucleotides encoding at least the mature form of the polypeptide having the deduced amino acid sequence as shown in SEQ ID NO:
- 12;
- (b) polynucleotides having the coding sequence, as shown in SEQ ID NO:
- 11 encoding at least the mature form of the polypeptide;
- (c) polynucleotides encoding a fragment or derivative of a polypeptide encoded by a polynucleotide of any one of (a) to (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to said polypeptide, and said fragment or derivative has bitter substance binding activity;
- (d) polynucleotides which are at least 50% identical to a polynucleotide as defined in any one of (a) to (c) and which code for a polypeptide having bitter substance binding activity;
- and
- (e) polynucleotides the complementary strand of which hybridizes under stringent conditions to a polynucleotide as defined in any one of (a) to (d) and which code for a polypeptide having bitter substance binding activity;
- comprising:
- (1) contacting said polypeptide or a host cell genetically engineered with said polynucleotide or with a vector containing said polynucleotide with a compound;
- (2) detecting the presence of the compound which binds to said polypeptide;
- and
- (3) determining whether the compound binds said polypeptide.
10 more elements
A process for isolating an antagonist of the bitter taste receptor activity of the polypeptide encoded by a polynucleotide selected from the group consisting of:
- (a) polynucleotides encoding at least the mature form of the polypeptide having the deduced amino acid sequence as shown in SEQ ID NO:
- 12;
- (b) polynucleotides having the coding sequence, as shown in SEQ ID NO:
- 11 encoding at least the mature form of the polypeptide;
- (c) polynucleotides encoding a fragment or derivative of a polypeptide encoded by a polynucleotide of any one of (a) to (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to said polypeptide, and said fragment or derivative has bitter taste receptor activity;
- (d) polynucleotides which are at least 50% identical to a polynucleotide as defined in any one of (a) to (c) and which code for a polypeptide having bitter taste receptor activity;
- and
- (e) polynucleotides the complementary strand of which hybridizes under stringent conditions to a polynucleotide as defined in any one of (a) to (d) and which code for a polypeptide having bitter taste receptor activity;
- comprising:
- (1) contacting said polypeptide or a host cell genetically engineered with said polynucleotide or with a vector containing said polynucleotide with a potential antagonist;
- and
- (2) determining whether the potential antagonist antagonizes the bitter taste receptor activity of said polypeptide.
9 more elements
A method for treating an abnormally increased or decreased sensitivity towards a bitter substance wherein said method comprises administering, to a subject in need of such treatment, one or more agents selected from the group consisting of:
- 1) A polynucleotide selected from the group consisting of:
- (a) polynucleotides encoding at least the mature form of the polypeptide having the deduced amino acid sequence as shown in SEQ ID NO:
- 12;
- (b) polynucleotides having the coding sequence, as shown in SEQ ID NO:
- 11 encoding at least the mature form of the polypeptide;
- (c) polynucleotides encoding a fragment or derivative of a polypeptide encoded by a polynucleotide of any one of (a) to (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to said polypeptide, and said fragment or derivative has bitter substance binding activity;
- (d) polynucleotides which are at least 50% identical to a polynucleotide as defined in any one of (a) to (c) and which code for a polypeptide having bitter substance binding activity;
- and
- (e) polynucleotides the complementary strand of which hybridizes under stringent conditions to a polynucleotide as defined in any one of (a) to (d) and which code for a polypeptide having bitter substance binding activity;
- or the complementary strand of such a polynucleotide;
- 2) a vector comprising a polynucleotide of part 1) above;
- 3) an antibody that specifically binds to a polypeptide that is encoded by a polynucleotide of part 1) above and which is obtainable by culturing a host cell genetically engineered to comprise a polynucleotide of part 1) above, and recovering the polypeptide encoded by said polynucleotide;
- and
- 4) an antagonist/inhibitor against the polypeptide of part 3) above, which is an antibody, the extracellular domain of the polypeptide of part 3) above, or a fragment thereof, or an inhibiting RNA.
11 more elements
Dependencies are inferred from the claim text.