Compositions Comprising Modified Collagen and Uses Therefor
AssigneeThe Johns Hopkins University
InventorsMichael Yu, Jennifer H. Elisseeff, Allen Y. Wang, Hyeseung J. Lee, Xiao Mo
Life of this patent
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Abstract
The invention provides modified collagen and related therapeutic and diagnostic methods.
Claims43
A method for modifying collagen, the method comprising contacting collagen with a collagen mimetic peptide, under conditions that provide for a physical interaction between the collagen and the collagen mimetic peptide.
3-7. (canceled)
9-24. (canceled)
A collagen mimetic peptide conjugate, wherein the conjugate is selected from the group consisting of an antibiotic, a cell adhesion molecule, a contrast agent, a detectable label, a growth factor, a component of the extracellular matrix, an anti-inflammatory, a polymer, PEG, and a small molecule, and wherein the collagen mimetic peptide comprises a Z-[X—Y-Gly]n repeat unit, wherein Z is any amino acid, X is proline or modified proline, Y is proline or modified proline, and n is an integer between 1 and 20, or wherein the peptide comprises an amino acid sequence selected from the group consisting of:
- Gly3-(ProHyPGly)6,
- Gly3-(ProHyPGly)7,
- Gly3-(ProHypGly)8,
- Gly3-(ProHypGly)9
- (ProHypGly)6-Tyr
- (ProHypGly)7-Tyr
- (ProHypGly)8-Tyr
- Cys-(Pro-Hyp-Gly)3,
- Cys-(Pro-Hyp-Gly)5, and
- Cys-(Pro-Hyp-Gly)7.
27-43. (canceled)
The collagen mimetic peptide conjugate of claim 43, wherein the peptide comprises:
- carboxyfluorescein-Gly3-(Pro-Hyp-Gly)6
- carboxyfluorescein-Gly3-lys-(Pro-Hyp-Gly)8
- PEG2000-Gly3-(Pro-Hyp-Gly)7
- Methoxy PEG2000-Gly3-(Pro-Hyp-Gly)7
- (Pro-Hyp-Gly)8-Gly3-PEG5000-OH
- [AcGly-Gly2-Lys-Gly3-(Pro-Hyp-Gly)8]4-star shaped PEG
- [CF-Gly3-Lys-Gly3-(Pro-Hyp-Gly)8]4-star shaped PEG
- AcGly-Gly2-Lys-Gly3-(Pro-Hyp-Gly)8,
- FL-PEG2000-Gly3-(Pro-Hyp-Gly)7, and
- 5 carboxyfluorescein-Gly3-(Pro-Hyp-Gly)8-Gly3-PEG5000-OH.
45-56. (canceled)
A method of modifying the adhesiveness of a composition, the method comprising contacting the composition with a collagen mimetic peptide (CMP) conjugate of claim 45.
58-62. (canceled)
64-75. (canceled)
A transmission electron microscopy marker comprising the nanoparticle of claim 62.
(canceled)
79-84. (canceled)
A method for diagnosing a subject as having or having a propensity to develop a disease characterized by disruption of a collagen structure, the method comprising:
- a) contacting a patient sample with a detectable CMP-conjugate;
- b) detecting an alteration in a collagen structure present in the patient sample, wherein the alteration diagnoses the subject as having or having a propensity to develop a disease characterized by disruption of a collagen structure.
A method for diagnosing a subject as having or having a propensity to develop a thrombosis, the method comprising:
- a) contacting a blood vessel with a detectable CMP-conjugate;
- b) detecting an alteration present in the blood vessel, wherein the alteration diagnoses the subject as having or having a propensity to develop a thrombosis.
87-94. (canceled)
A composition for repelling cell adhesion, the composition comprising a CMP-poly(ethylene glycol) conjugate.
96-100. (canceled)
A matrix comprising a CMP-polymer conjugate, wherein the CMP-polymer conjugate alters the adhesive properties of the matrix.
102-109. (canceled)
A method for repelling cell adhesion on a composition, the method comprising fixing a CMP-PEG conjugate to a composition, wherein the CMP-PEG conjugate repels cell adhesion on the composition.
111-123. (canceled)
A composition for crosslinking collagen, the composition comprising a CMP-poly(ethylene glycol) conjugate.
125-129. (canceled)
A method for preventing or treating thrombosis in a subject, the method comprising contacting a blood vessel having or having a propensity to develop a thrombosis with a CMP-anti-thrombosis conjugate;
- wherein the contacting prevents or treats a thrombosis.
131-136. (canceled)
A diagnostic marker that detects a vessel having or having an increased propensity to develop a thrombosis relative to a control vessel, the marker comprising a detectable CMP conjugate that binds collagen.
138-154. (canceled)
156-175. (canceled)
A matrix comprising a polymer in association with a collagen mimetic peptide, wherein the matrix promotes the survival, differentiation, or proliferation of a cell.
177-195. (canceled)
A method for synthesizing a matrix, the method comprising contacting a collagen mimetic peptide to a polymer and cross-linking the polymer such that a molecular matrix is formed.
197-203. (canceled)
A method for promoting cell survival or proliferation, comprising growing a cell in contact with a matrix comprising a polymer and a collagen mimetic peptide.
205-219. (canceled)
A method for replacing a damaged or absent tissue, the method comprising
- a) growing a cell in a matrix of claim 170;
- and
- b) contacting a damaged tissue or site of an absent tissue with the cell and matrix of step (a), such that the cell and matrix replaces the damaged or absent tissue.
221-228. (canceled)
Dependencies are inferred from the claim text.