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US 2008/0286807 A1Published application
Methods and Reagents for Elimination or Reduction of False Positives in the Analysis of a Sample
AssigneeOregon Health & Science University
InventorAntony Bakke
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Abstract
Methods, apparatuses, and systems for eliminating or reducing false positives in assays are provided. More specifically, there is provided a method to absorb a false positive signal with an absorber in favor of detecting a true positive signal. Embodiments of the present invention include but are not limited to systems and methods for detecting antibodies with greater specificity than available assays and also for detecting a greater variety of antibodies.
Claims31
A method of measuring a positive receptor target in a sample, comprising:
- providing a sample having a substrate, said substrate having a positive receptor target including at least one binding site and a false positive receptor including at least two binding sites;
- contacting the sample with a fluorescent probe adapted to bind to said at least one binding site of said positive receptor target and nonspecifically to at least one of said at least two binding sites of said false positive receptor, wherein when said fluorescent probe is bound to a binding site of said positive receptor target, said fluorescent probe fluoresces;
- and
- contacting the sample with an absorber construct adapted to bind to at least one of said at least two binding sites of said false positive receptor, wherein when said absorber construct is bound to a binding site of said false positive receptor and when said fluorescent probe is bound to an adjacent binding site on said false positive receptor, the fluorescence of said fluorescent probe is absorbed, at least partially, by said absorber construct.
1 more element
A kit, comprising:
- a fluorescent probe adapted to bind to at least one binding site of a positive receptor target and nonspecifically to at least one of at least two binding sites of a false positive receptor;
- and
- an absorber construct adapted to bind to at least one of said at least two binding sites of said false positive receptor, wherein said absorber construct is adapted to absorb fluorescence, at least partially, from said fluorescent probe when said fluorescent probe and said absorber construct are bound to adjacent binding sites on said false positive receptor.
Dependencies are inferred from the claim text.