Dispensing Device For Microfluidic Droplets Especially For Cytometry
OwnerPhilippe Renaud, Nathalie Picollet-D'Hahan +2inventors
InventorsPhilippe Renaud, Nathalie Picollet-D'Hahan, Vincent Haguet, Francois Chatelain
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Abstract
The invention relates to a dispensing device for droplets comprising a first channel (8, 10), known as main channel, for circulation of a first fluid flow, a second channel (12, 13) for circulation of fluid, which forms with the first channel an intersection zone (27) and terminates in an ejection orifice (20), means (4) for measuring a physical property particles or cells in the first channel (18), and means for engendering a pressure wave in the second channel (12, 13).
Claims42
Device for dispensing droplets comprising:
- a first channel, known as main channel, comprising at least one branch, for circulation of a first fluid flow,
- a second channel for circulation of fluid, comprising at least one branch, which forms an intersection zone with the first channel and terminates via an ejection orifice,
- measuring means of a physical property of particles or cells in the first channel, and
- means for generating a pressure wave in the second channel.
Method for dispensing droplets, making use of a first channel and a second channel which forms an intersection zone with the first channel and terminates in an ejection orifice, the process comprising the steps consisting of:
- circulating a first fluid in the first channel;
- measuring a physical property or analysing the contents of the flow of the first fluid;
- and,
- generating a pressure wave as depending on the results of the preceding step.
Device for dispensing droplets comprising:
- a first channel, known as main channel, comprising two branches, with an input opening for circulation of a first fluid flow,
- a second channel with an injection opening for circulation of a second fluid, comprising branches on either side of the main channel, and which terminates via an ejection orifice for part of the flow and another outlet orifice for another part of flow,
- the branches of the first and second channel joining up in an intersection zone,
- a series of electrodes for measuring a physical property of particles or cells in the first channel, matching electrodes being arranged on either side of a channel branch, and
- means for generating a pressure wave in the second channel.
Dependencies are inferred from the claim text.