Generation of plants with altered oil, protein, or fiber content
AssigneeAgrigenetics, Inc.
InventorsJohn P. Davies, Hein Tsoeng (Medard) Ng
Life of this patent
Estimating the term…
Abstract
The present invention is directed to plants that display an improved oil quantity phenotype or an improved meal quality phenotype due to altered expression of an HIO nucleic acid. The invention is further directed to methods of generating plants with an improved oil quantity phenotype or improved meal quality phenotype.
Claims29
A transgenic plant, comprising a plant transformation vector comprising a nucleotide sequence that encodes or is complementary to a sequence that encodes an HIO polypeptide comprising the amino acid sequence as set forth in SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:
- 16, or SEQ ID NO:
- 18, or an ortholog or paralog thereof, whereby the transgenic plant has has an increased oil content phenotype or an increased meal quality phenotype, relative to control plants.
A method of producing increased oil content in a plant, said method comprising:
- a) introducing into progenitor cells of the plant a plant transformation vector comprising a nucleotide sequence that encodes or is complementary to a sequence that encodes a HIO polypeptide comprising the amino acid sequence as set forth in SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:
- 16, or SEQ ID NO:
- 18, or an ortholog or paralog thereof, and
- b) growing the transformed progenitor cells to produce a transgenic plant, wherein said polynucleotide sequence is expressed, and said transgenic plant exhibits an increased oil content phenotype relative to control plants.
A method of generating a plant having an increased oil content, comprising identifying a plant that has an allele in its HIO gene that results in increased oil content compared to plants lacking the allele and generating progeny of said identified plant, wherein the generated progeny inherit the allele and have the high oil phenotype.
A feed, meal, grain, food, or seed comprising a polypeptide encoded by the nucleic acid sequence as set forth in SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:
- 13, SEQ ID NO:
- 15, or SEQ ID NO:
- 17.
A feed, meal, grain, food, or seed comprising a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:
- 16, or SEQ ID NO:
- 18, or an ortholog or paralog thereof.
A method of producing an improved meal quality phenotype in a plant, said method comprising:
- a) introducing into progenitor cells of the plant a plant transformation vector comprising a nucleotide sequence that encodes or is complementary to a sequence that encodes an HIO polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:
- 16, or SEQ ID NO:
- 18, or an ortholog or paralog thereof, and
- b) growing the transformed progenitor cells to produce a transgenic plant, wherein the nucleotide sequence is expressed, and the transgenic plant exhibits an increased meal quality phenotype relative to control plants,
- thereby producing the improved meal quality phenotype in the plant.
A method of generating a plant having an improved meal quality phenotype comprising identifying a plant that has an allele in its HIO gene that results in increased meal quality, compared to plants lacking the allele, and generating progeny of said identified plant, wherein the generated progeny inherit the allele and have the increased meal quality,
- thereby generating a plant having an improved meal quality phenotype.
Dependencies are inferred from the claim text.