# US 2008/0189807 A1 · Nitrogen Transport Metabolism

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- Number: US 2008/0189807 A1
- Document ID: US20080189807A1
- Status: Published application
- Kind: A1
- Application number: 12/098,487
- Filed: 2008-04-07
- Published: 2008-08-07
- Earliest claimed: 1998-08-28
- Assignees: E.I. du Pont de Nemours and Company
- Inventors: Stephen M. Allen; J. Antoni Rafalski
- Web page: https://patentgrep.com/patent/US20080189807A1

## CPC

- C12N 15/8261: {with agronomic (input) traits, e.g. crop yield}
- C07K 14/415: from plants
- C07K 2319/00: Fusion polypeptide
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

This invention relates to an isolated nucleic acid fragments encoding an ammonium transporter. The invention also relates to the construction of a recombinant DNA constructencoding all or a portion of ammonium transporters, in sense or antisense orientation, wherein expression of the recombinant DNA construct may alter levels of the ammonium transporter in a transformed host cell.

## Claims

### Claim at position 1

-10. (canceled)

### Claim 11

An isolated polynucleotide comprising:
(a) a nucleotide sequence encoding a polypeptide having ammonium transporter activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:14 have at least 90% sequence identity based on the Clustal alignment method, or
(b) the complement of the nucleotide sequence, wherein the complement and the nucleotide sequence contain the same number of nucleotides and are 100% complementary.

### Claim 12 (depends on 11)

The polynucleotide of claim 11 wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:14
have at least 95% sequence identity based on the Clustal alignment method.

### Claim 13 (depends on 11)

The polynucleotide of claim 11 wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:14.

### Claim 14 (depends on 11)

The polynucleotide of claim 11 wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO:13

### Claim 15 (depends on 11)

A vector comprising the polynucleotide of claim 11.

### Claim 16 (depends on 11)

A recombinant DNA construct comprising the polynucleotide of claim 11 operably linked to a regulatory sequence.

### Claim 17 (depends on 11)

A method for transforming a cell comprising transforming a cell with the polynucleotide of claim 11.

### Claim 18 (depends on 16)

A cell comprising the recombinant DNA construct of claim 16.

### Claim 19 (depends on 11)

A method for producing a plant comprising transforming a plant cell with the polynucleotide of claim 11 and regenerating a plant from the transformed plant cell.

### Claim 20 (depends on 16)

A plant comprising the recombinant DNA construct of claim 16.

### Claim 21 (depends on 16)

A seed comprising the recombinant DNA construct of claim 16.