# US 2008/0189806 A1 · Protein kinase stress-related proteins and methods of use in plants

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- Number: US 2008/0189806 A1
- Document ID: US20080189806A1
- Status: Published application
- Kind: A1
- Application number: 11/961,496
- Filed: 2007-12-20
- Published: 2008-08-07
- Earliest claimed: 2000-04-07
- Assignees: BASF Plant Science GmbH
- Inventors: Oswaldo da Costa e Silva; Hans J. Bohnert; Nocha van Thielen; Ruoying Chen; Rodrigo Sarria-Millan
- Web page: https://patentgrep.com/patent/US20080189806A1

## CPC

- C12N 15/8273: {for drought, cold, salt resistance}
- C07K 14/415: from plants
- C12N 9/1205: {Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases}
- C12N 9/16: acting on ester bonds (3.1)
- C12N 15/8217: {Gene switch}
- A01K 2217/05: Animals comprising random inserted nucleic acids (transgenic)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS

## Abstract

A transgenic plant transformed by a Protein Kinase Stress-Related Protein (PKSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated PKSRPs, and isolated nucleic acid coding PKSRPs, and vectors and host cells containing the latter.

## Claims

### Claim 1

A transgenic plant cell transformed with an expression vector comprising an isolated polynucleotide selected from the group consisting of:
a) a polynucleotide having a sequence comprising nucleotides 1 to 1731 of SEQ ID NO:23;
b) a polynucleotide having a sequence comprising nucleotides 1 to 1407 of SEQ ID NO:24; and
c) a polynucleotide encoding a polypeptide having a sequence as set forth in amino acids 1 to 346 of SEQ ID NO:36.

### Claim 2 (depends on 1)

The plant cell of claim 1, wherein the polynucleotide has the sequence comprising nucleotides 1 to 1731 of SEQ ID NO:23.

### Claim 3 (depends on 1)

The plant cell of claim 1, wherein the polynucleotide has the sequence comprising nucleotides 1 to 1407 of SEQ ID NO:24.

### Claim 4 (depends on 1)

The plant cell of claim 1, wherein the polynucleotide encodes the polypeptide having the sequence comprising amino acids 1 to 346 of SEQ ID NO:36.

### Claim 5

A transgenic plant transformed with an expression cassette comprising an isolated polynucleotide selected from the group consisting of:
a) a polynucleotide having a sequence comprising nucleotides 1 to 1731 of SEQ ID NO:23;
b) a polynucleotide having a sequence comprising nucleotides 1 to 1407 of SEQ ID NO:24; and
c) a polynucleotide encoding a polypeptide having a sequence as set forth in amino acids 1 to 346 of SEQ ID NO:36.

### Claim 6 (depends on 5)

The plant of claim 5, wherein the polynucleotide has the sequence comprising nucleotides 1 to 1731 of SEQ ID NO:23.

### Claim 7 (depends on 5)

The plant of claim 5, wherein the polynucleotide has the sequence comprising nucleotides 1 to 1407 of SEQ ID NO:24.

### Claim 8 (depends on 5)

The plant of claim 5, wherein the polynucleotide encodes the polypeptide having the sequence comprising amino acids 1 to 346 of SEQ ID NO:36.

### Claim 9 (depends on 5)

The plant of claim 5, wherein the plant is a monocot.

### Claim 10 (depends on 5)

The plant of claim 5, wherein the plant is a dicot.

### Claim 11 (depends on 5)

The plant of claim 5, wherein the plant is selected from the group consisting of maize, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, rapeseed, canola, manihot, pepper, sunflower, tagetes, potato, tobacco, eggplant, tomato, Vicia species, pea, alfalfa, coffee, cacao, tea, Salix species, oil palm, coconut, perennial grasses, and a forage crop plant.

### Claim 12 (depends on 11)

The plant of claim 11, which is maize.

### Claim 13 (depends on 11)

The plant of claim 11, which is soybean.

### Claim 14 (depends on 11)

The plant of claim 11, which is rapeseed or canola.

### Claim 15 (depends on 11)

The plant of claim 11, which is cotton.

### Claim 16

A seed which is true breeding for a transgene comprising a polynucleotide selected from the group consisting of:
a) a polynucleotide having a sequence comprising nucleotides 1 to 1731 of SEQ ID NO:23;
b) a polynucleotide having a sequence comprising nucleotides 1 to 1407 of SEQ ID NO:24; and
c) a polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 346 of SEQ ID NO:36.

### Claim 17 (depends on 16)

The seed of claim 16, wherein the polynucleotide has the sequence comprising nucleotides 1 to 1731 of SEQ ID NO:23.

### Claim 18 (depends on 16)

The seed of claim 16, wherein the polynucleotide has the sequence comprising nucleotides 1 to 1407 of SEQ ID NO:24.

### Claim 19 (depends on 16)

The seed of claim 16, wherein the polynucleotide encodes the polypeptide having the sequence comprising amino acids 1 to 346 of SEQ ID NO:36.

### Claim 20

An isolated nucleic acid comprising a polynucleotide selected from the group consisting of:
a) a polynucleotide having a sequence comprising nucleotides 1 to 1731 of SEQ ID NO:23;
b) a polynucleotide having a sequence comprising nucleotides 1 to 1407 of SEQ ID NO:24; and
c) a polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 346 of SEQ ID NO:36.

### Claim 21 (depends on 20)

The isolated nucleic acid of claim 20, wherein the polynucleotide has the sequence comprising nucleotides 1 to 1731 of SEQ ID NO:23.

### Claim 22 (depends on 20)

The isolated nucleic acid of claim 20, wherein the polynucleotide has the sequence comprising nucleotides 1 to 1407 of SEQ ID NO:24.

### Claim 23 (depends on 20)

The isolated nucleic acid of claim 20, wherein the polynucleotide encodes the polypeptide having the sequence comprising amino acids 1 to 346 of SEQ ID NO:36.

### Claim 24

A method of producing a drought-tolerant transgenic plant, the method comprising the steps of:
a) transforming a plant cell with an expression vector comprising a polynucleotide selected from the group consisting of:
a) a polynucleotide having a sequence comprising nucleotides 1 to 1731 of SEQ ID NO:23;
b) a polynucleotide having a sequence comprising nucleotides 1 to 1407 of SEQ ID NO:24; and
c) a polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 346 of SEQ ID NO:36.

### Claim 25 (depends on 24)

The method of claim 24, wherein the polynucleotide has the sequence comprising nucleotides 1 to 1731 of SEQ ID NO:23.

### Claim 26 (depends on 24)

The method of claim 24, wherein the polynucleotide has the sequence comprising nucleotides 1 to 1407 of SEQ ID NO:24.

### Claim 27 (depends on 24)

The method of claim 24, wherein the polynucleotide encodes the polypeptide having the sequence comprising amino acids 1 to 346 of SEQ ID NO:36.