# US 2008/0188564 A1 · Insecticidal and Nematicidal Compositions and Methods of Use > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2008/0188564 A1 - Document ID: US20080188564A1 - Status: Published application - Kind: A1 - Application number: 11/720,117 - Filed: 2005-11-29 - Published: 2008-08-07 - Earliest claimed: 2004-11-30 - Inventors: Jeffrey R. Bloomquist; Edwin E. Lewis - Web page: https://patentgrep.com/patent/US20080188564A1 ## CPC - A01N 31/08: Oxygen or sulfur directly attached to an aromatic ring system - A01N 27/00: Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons - A01N 47/46: containing —N=C=S groups - A61P 33/00: Antiparasitic agents - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS ## Abstract The present invention provides methods of protecting plants and other multicellular organisms from the parasitic activity of nematodes and insects. The methods use compounds, such as stilbene compounds, as nematicidal and insecticidal compounds, which act to alter the activity of one or more anion transporters in the nematodes and insects. Methods of screening for such compounds are also provided. ## Claims ### Claim 1 A method of treating a multicellular host organism attacked, bitten, infected, or parasitized by at least one nematode, at least one insect, at least one acarine, or combinations of two or all three of these, said method comprising contacting the host organism or its environment with at least one compound in amount sufficient to disrupt the activity of at least one anion transporter of the nematode, insect, or acarine, the disruption reducing the viability or activity of the nematode, insect, or acarine. ### Claim 2 (depends on 1) The method of claim 1, wherein the multicellular organism is a plant. ### Claim 3 (depends on 2) The method of claim 2, wherein the plant is a food crop. ### Claim 4 (depends on 1) The method of claim 1, wherein the multicellular organism is an animal. ### Claim 5 (depends on 4) The method of claim 4, wherein the animal is a mammal. ### Claim 6 (depends on 5) The method of claim 5, wherein the mammal is a farm animal. ### Claim 7 (depends on 1) The method of claim 1, wherein the compound is a stilbene compound. ### Claim 8 A method of protecting a multicellular host organism attacked, bitten, infected, or parasitized by at least one nematode, insect, or acarine, said method comprising contacting the nematode or insect or acarine with at least one compound in amount sufficient to disrupt the activity of at least one anion transporter of the nematode, insect, or acarine, the disruption reducing the activity or viability of the nematode or insect or acarine. ### Claim 9 (depends on 8) The method of claim 8, wherein the multicellular organism is a plant. ### Claim 10 (depends on 9) The method of claim 9, wherein the plant is a food crop. ### Claim 11 (depends on 8) The method of claim 8, wherein the multicellular organism is an animal. ### Claim 12 (depends on 11) The method of claim 11, wherein the animal is a mammal. ### Claim 13 (depends on 12) The method of claim 12, wherein the mammal is a farm animal. ### Claim 14 (depends on 8) The method of claim 8, wherein the compound is a stilbene compound. ### Claim 15 A method of identifying a test compound having nematicidal, insecticidal, or acaricidal activity, said method comprising: exposing at least one anion transporter from at least one nematode, insect, or Acari to at least one test compound, providing sufficient time for the anion transporter(s) and test compound(s) to come into contact; and determining whether one or more of the test compounds affected the activity of one or more of the anion transporters, wherein an effect on the activity of at least one anion transporter indicates nematicidal or insecticidal or acaricidal activity of at least one of the test compounds. ### Claim 16 (depends on 15) The method of claim 15, wherein the method is practiced, at least partially, in vitro. ### Claim 17 (depends on 15) The method of claim 15, wherein at least one of the test compounds is a stilbene compound. ### Claim 18 (depends on 15) The method of claim 15, wherein determining comprises measuring the activity of at least on anion transporter that was exposed to at least one test compound. ### Claim 19 (depends on 15) The method of claim 15, further comprising determining the activity of the anion transporters under the same conditions, but without the presence of the test compound(s). ### Claim 20 (depends on 15) The method of claim 15, further comprising identifying the test compound(s) showing an effect on the anion transporter(s). ### Claim 21 (depends on 15) The method of claim 15, further comprising repeating the exposing, providing, and determining steps more than one time, wherein each iteration of the steps is performed using fewer test compound(s), fewer AT, or fewer of both. ### Claim 22 (depends on 15) The method of claim 15, comprising determining the effect of a single test compound on a single AT. ### Claim 23 (depends on 15) The method of claim 15, further comprising correlating the activity of the test compound(s) on inhibition of growth or killing of at least one nematode, insect, or acarine.