# US 2008/0187988 A1 · Cytoplasmic antigens for detection of candida

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- Number: US 2008/0187988 A1
- Document ID: US20080187988A1
- Status: Published application
- Kind: A1
- Application number: 11/928,632
- Filed: 2007-10-30
- Published: 2008-08-07
- Earliest claimed: 2001-04-25
- Assignees: Rockeby Biomed (Australia) Pty. Ltd.
- Inventors: John Warmington; Denis Ballantyne
- Web page: https://patentgrep.com/patent/US20080187988A1

## CPC

- G01N 33/56961: {Plant cells or fungi}
- A61P 31/10: Antimycotics
- C07K 14/40: from Candida
- G01N 33/571: for venereal disease, e.g. syphilis, gonorrhoea {(herpes G01N33/56994; chlamydia G01N33/56927)}
- G01N 2333/40: from Candida
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention relates to a method and a means of diagnosing Candida infection. In particular the present invention relates to a method of diagnosing Candida infection by measuring the levels of antibody to Candida cytoplasmic antigen present in a biological sample taken from a subject at risk of, or suspected to be suffering from a Candida infection.

## Claims

### Claim 1

A method of preparing a Candida antigen composition comprising the steps of:
(a) providing Candida albicans yeast cells;
(b) disrupting said yeast cells to produce a cell lysate;
(c) clarifying said cell lysate to produce a soluble cytoplasmic antigen fraction;
(d) filtering and organically extracting said soluble cytoplasmic antigen fraction; and
(e) depleting soluble cell wall mannoprotein from said soluble cytoplasmic antigen fraction, to produce a soluble cytoplasmic antigen fraction comprising antigens of molecular weights 55 kDa, 30 kDa and 20 kDa.

### Claim 2 (depends on 1)

The method of claim 1, wherein the yeast cells are disrupted by mechanical disruption.

### Claim 3 (depends on 1)

The method of claim 1, wherein step (c) is performed by centrifugation.

### Claim 4 (depends on 1)

The method of claim 1, wherein the filtration of step (d) is performed by using a filter that has a 0.45 μm filter membrane.

### Claim 5 (depends on 1)

The method of claim 1, wherein the organic extraction of step (d) is a chloroform extraction.

### Claim 6 (depends on 1)

The method of claim 1, wherein step (e) is performed by Con A-Sepharose chromatography.