# US 2008/0187979 A1 · Method for Preparing Sample Solution and Sample Solution Preparing Apparatus > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2008/0187979 A1 - Document ID: US20080187979A1 - Status: Published application - Kind: A1 - Application number: 11/793,314 - Filed: 2006-01-31 - Published: 2008-08-07 - Earliest claimed: 2005-01-31 - Inventors: Toshihiro Mori - Web page: https://patentgrep.com/patent/US20080187979A1 ## CPC - C12N 15/1003: {Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor} - C12N 15/1006: {by means of a solid support carrier, e.g. particles, polymers} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract A method for preparing a sample solution, which is a step preceding a process for separating and purifying a nucleic acid by extracting a nucleic acid from the sample solution, the method comprising: injecting a sample solution into a container for preparation; subjecting the sample solution to a treatment of agitation by shaking in which the sample solution is agitated by applying a light vibration to the container; and subjecting the sample solution to a treatment of agitation by pipetting in which the sample solution is agitated by pipetting the sample solution in the container. ## Claims ### Claim 1 A method for preparing a sample solution, which is a step preceding a process for separating and purifying a nucleic acid by extracting a nucleic acid from the sample solution, the method comprising: injecting a sample solution into a container for preparation; subjecting the sample solution to a treatment of agitation by shaking in which the sample solution is agitated by applying a light vibration to the container; and subjecting the sample solution to a treatment of agitation by pipetting in which the sample solution is agitated by pipetting the sample solution in the container. ### Claim 2 (depends on 1) The method for preparing a sample solution according to claim 1, wherein the treatment of agitation by shaking involves an agitation by rotatory shaking, and a speed of the rotation is in a range of from 400 to 2000 rpm. ### Claim 3 (depends on 1) The method for preparing a sample solution according to claim 1, wherein the treatment of agitation by pipetting is carried out in a manner that a volume for one pipetting is in a range of from 50 to 1000 μl. ### Claim 4 (depends on 1) The method for preparing a sample solution according to claim 1, wherein the treatment of agitation by pipetting is carried out in a manner that a number of a repetition of the pipetting is in a range of from 10 to 100 times. ### Claim 5 (depends on 1) The method for preparing a sample solution according to claim 1, which involves a simultaneous treatment of a plurality of the sample solutions injected into the container. ### Claim 6 (depends on 1) The method for preparing a sample solution according to claim 1, wherein the process for injecting the sample solution into the container for preparation involves a process of adding a proteolytic enzyme, a sample containing a nucleic acid and a pretreatment solution containing at least one selected from a chaotropic salt, a surfactant, a defoaming agent, a nucleic acid stabilizer and a buffer, and wherein the proteolytic enzyme, the sample and the pretreatment solution are added in this order, the pretreatment solution, the sample and the proteolytic enzyme are added in this order, or the sample, the pretreatment solution and the proteolytic enzyme are added in this order. ### Claim 7 (depends on 6) The method for preparing a sample solution according to claim 6, wherein the process for injecting the sample solution into the container for preparation involves a further addition of a water-soluble organic solvent, after adding the proteolytic enzyme, the sample and the pretreatment solution. ### Claim 8 (depends on 6) The method for preparing a sample solution according to claim 6, wherein the sample solution is obtained by preparing a whole blood. ### Claim 9 (depends on 7) The method for preparing a sample solution according to claim 7, wherein the water-soluble organic solvent comprises at least one selected from methanol, ethanol, propanol and butanol. ### Claim 10 (depends on 7) The method for preparing a sample solution according to claim 7, wherein the sample solution is contacted with a nucleic acid adsorbing solid phase after the addition of a water-soluble organic solvent. ### Claim 11 (depends on 10) The method for preparing a sample solution according to claim 10, wherein the solid phase is in a membrane form. ### Claim 12 (depends on 10) The method for preparing a sample solution according to claim 10, wherein the solid phase comprises a silica or a derivative thereof, a diatomaceous earth or an alumina. ### Claim 13 (depends on 10) The method for preparing a sample solution according to claim 10, wherein the solid phase comprises an organic polymer. ### Claim 14 (depends on 13) The method for preparing a sample solution according to claim 13, wherein the organic polymer is an organic polymer having a polysaccharide structure. ### Claim 15 (depends on 13) The method for preparing a sample solution according to claim 13, wherein the organic polymer is an acetylcellulose. ### Claim 16 (depends on 13) The method for preparing a sample solution according to claim 13, wherein the organic polymer is an organic polymer obtained by a saponification of an acetylcellulose or a mixture of acetylcelluloses different from each other in acetyl value. ### Claim 17 (depends on 13) The method for preparing a sample solution according to claim 13, wherein the organic polymer is a regenerated cellulose. ### Claim 18 A sample solution preparing apparatus for preparing a sample solution containing a nucleic acid at a step preceding a process for separating and purifying a nucleic acid by extracting a nucleic acid from the sample solution, the sample solution preparing apparatus comprising: a container for preparation into which the sample solution is injected; an agitation by shaking means for agitating the sample solution by applying a light vibration to the container; and an agitation by pipetting means for agitating the sample solution by pipetting the sample solution in the container.