# US 2008/0187959 A1 · Sulfotransferase, peptide thereof and dna encoding the same

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- Number: US 2008/0187959 A1
- Document ID: US20080187959A1
- Status: Published application
- Kind: A1
- Application number: 11/748,403
- Filed: 2007-05-14
- Published: 2008-08-07
- Earliest claimed: 2002-03-04
- Assignees: Seikagaku Kogyo Kabushiki Kaisha
- Inventors: Hisashi Narimatsu; Shigemi Sugioka; Hideo Mochizuki
- Web page: https://patentgrep.com/patent/US20080187959A1

## CPC

- C12N 9/13: {transferring sulfur containing groups (2.8)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A glycosaminoglycan sulfotransferase, a peptide thereof, a nucleic acid comprising a nucleotide sequence encoding the same, an enzyme agent for the synthesis of a glycosaminoglycan, which comprises the above-described enzyme or polypeptide, and a process for producing a glycosaminoglycan, which uses the enzyme agent.

## Claims

### Claim at position 1

1-5. (canceled)

### Claim 6

A nucleic acid selected from the group consisting of (I), (II) and (III):
(I) a nucleic acid which encodes:
a polypeptide which comprises amino acid numbers 37 to 346 in the amino acid sequence of SEQ ID NO:2, or
a sulfotransferase which comprises a polypeptide which comprises amino acid numbers 37 to 346 in the amino acid sequence of SEQ ID NO:2 and has activity of transferring a sulfate group from a sulfate group donor to a glycosaminoglycan which is a sulfate group acceptor;
(II) a nucleic acid which consists of the nucleotide sequence of SEQ ID NO:1 and
(III) a nucleic acid which hybridizes, under stringent conditions, with:
the nucleic acid according to (I) or (II) or a nucleic acid which consists of the nucleotide sequence of SEQ ID NO:1 or
a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence of the nucleic acid according to (I) or (II) or the nucleotide sequence of SEQ ID NO:1.

### Claim 7

(canceled)

### Claim 8

(canceled)

### Claim 9 (depends on 6)

An expression vector which comprises the nucleic acid according to claim 6.

### Claim 10 (depends on 9)

A recombinant which comprises the expression vector according to claim 9.

### Claim 11 (depends on 9)

A recombinant which comprises a host cell into which the expression vector according to claim 9 is introduced.

### Claim 12 (depends on 9, 10, 11)

A process for producing a polypeptide or a sulfotransferase, which comprises:
growing a recombinant which comprises the expression vector according to claim 10 or 11 or a recombinant which comprises a host cell into which the expression vector according to claim 9 is introduced, and
recovering a polypeptide which comprises amino acid numbers 37 to 346 in the amino acid sequence of SEQ ID NO:2 or a sulfotransferase which comprises the polypeptide and has activity of transferring a sulfate group from a sulfate group donor to a glycosaminoglycan which is a sulfate group acceptor from the obtained grown recombinant.

### Claim 13

(canceled)

### Claim 14

A process for producing a glycosaminoglycan comprising the structure of the following formula (1), which comprises reacting an enzyme agent with heparin or heparan sulfate to transfer a sulfate group from a sulfate group donor to a sulfate group acceptor:
wherein the enzyme agent comprises a polypeptide which comprises amino acid numbers 37 to 346 in the amino acid sequence of SEQ ID NO:2, or a sulfotransferase which comprises the polypeptide and has activity of transferring a sulfate group from a sulfate group donor to a glycosaminoglycan which is a sulfate group acceptor.

### Claim 15

(canceled)