# US 2008/0187599 A1 · Method and Apparatus for Pressure Sodding a Biological Vascular Conduit

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2008/0187599 A1
- Document ID: US20080187599A1
- Status: Published application
- Kind: A1
- Application number: 11/885,916
- Filed: 2006-03-08
- Published: 2008-08-07
- Earliest claimed: 2005-03-08
- Inventors: Paul J. Dimuzio; Patrick J. Greaney; Christopher A. Dimatteo
- Web page: https://patentgrep.com/patent/US20080187599A1

## CPC

- A61F 2/062: {Apparatus for the production of blood vessels made from natural tissue or with layers of living cells}
- A61F: FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS (dental prosthetics A61C)

## Abstract

The present invention is directed to a method for lining a biological vascular conduit with cells. The method utilizes a suitable biologic tube conduit with luminal characteristics that simulate exposed basement membrane to allow for cell attachment. The biologic conduit is secured within a seeding chamber. Cells are introduced into the conduit. Pressure is applied to the seeding chamber such that each end receives substantially equal pressure.

## Claims

### Claim 1

A method for lining a biologic vascular conduit with cells comprising the steps of:
(a) utilizing a suitable biologic tube conduit with luminal characteristics that simulate exposed basement membrane to allow for cell attachment;
(b) securing the biologic conduit within a seeding chamber;
(c) introducing cells into the conduit; and
(d) pressuring the seeding chamber at both ends, such that each end receives substantially equal pressure.

### Claim 2 (depends on 1)

The method of claim 1, wherein the biologic tube conduit is an acellular collagen scaffold or a decellularized vascular tissue graft.

### Claim 3 (depends on 1)

The method of claim 1, further comprising rotating the conduit along its axis.

### Claim 4 (depends on 1)

The method of claim 1, wherein the cells are microvessel derived endothelial cells, preadipocytes, fibroblasts, or mixed isolates.

### Claim 5 (depends on 1)

The method of claim 1, wherein the cells are isolated from a subject to receive the vascular conduit.

### Claim 6 (depends on 1)

The method of claim 1, further comprising adding a preservative into its chamber when the desired level of cell density was reached.

### Claim 7

An apparatus for pressure sodding a biologic vascular conduit comprising:
at least one graft treatment chamber having opposed ends;
an attachment member at each end of the treatment chamber to secure a biologic tube conduit within said treatment chamber; and
an adjustable pressure source located at each end of the treatment chamber,
wherein substantially equal pressure can be applied at each end of the conduit.

### Claim 8 (depends on 2)

The apparatus of claim 2, wherein the biologic tube conduit is movably disposed.