Methods for Improving Crop Plant Architecture and Yield
AssigneePioneer Hi-Bred International, Inc.
InventorsOlga N. Danilevskaya, Evgueni Ananiev, Carl R. Simmons, Pedro Hermon, Rajeev Gupta
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Abstract
The present invention provides methods for altering plant characteristics by introducing into plants, isolated nucleic acid molecules that can be used to produce transgenic plants characterized by altered plant architecture, plant maturity, carbon and nitrogen partitioning and or improved harvestable yield. Also provided are isolated nucleic acids that encode PDR polypeptides, vectors capable of expressing such nucleic acid molecules, host cells containing such vectors, and polypeptides encoded by such nucleic acids.
Claims60
A method for plant characteristics, the method comprising:
- a. introducing into a plant cell a recombinant expression cassette comprising a polynucleotide whose expression, alone or in combination with additional polynucleotides, functions as a plant developmental regulator polypeptide within the plant;
- b. culturing the plant cell under plant forming conditions to produce a plant;
- and,
- c. inducing expression of the polynucleotide for a time sufficient to alter the architecture of the plant.
A method for increasing plant harvestable yield, the method comprising:
- a. introducing into a plant cell a recombinant expression cassette comprising a polynucleotide whose expression, alone or in combination with additional polynucleotides, functions as a plant developmental regulator polypeptide within the plant;
- b. culturing the plant cell under plant forming conditions to produce a plant;
- and
- c. inducing expression of the polynucleotide for a time sufficient to increase the harvestable yield of the plant.
An isolated nucleic acid comprising a polynucleotide sequence of the PDR coding region or a complement thereof.
An isolated nucleic acid wherein the polynucleotide has at least 75% sequence identity to the PDR coding region as determined by GAP 10 analysis using default parameters over the entire length of the sequence, or a complement thereof, wherein expression of the polynucleotide modulates the level of PDR expression in a plant.
An isolated nucleic acid wherein the polynucleotide hybridizes under high stringency conditions to the PDR coding region, or a complement thereof, wherein expression of the polynucleotide modulates the level of PDR expression in a plant.
An isolated nucleic acid wherein the polynucleotide comprises the PDR coding region, or a variant thereof, wherein the expression of the variant modulates the level of PDR expression in a plant.
An isolated nucleic acid wherein the polynucleotide comprises a fragment of the PDR coding region.
An isolated nucleic acid comprising a polynucleotide sequence of the PDR coding region, or a complement thereof.
An isolated polynucleotide selected from the group consisting of:
- a. a polynucleotide having at least 80% sequence identity, as determined by the GAP algorithm under default parameters, to the full length sequence of a polynucleotide selected from the group consisting of SEQ ID NOS:
- 51, 89, 91, 97, 119, 127, 139, 147, 165 and 171;
- wherein the polynucleotide encodes a polypeptide that has PDR functions;
- and
- b. a polynucleotide encoding a polypeptide selected from the group consisting of SEQ ID NO:
- 50, 90, 92, 98, 120, 128, 140, 148, 166, and 170;
- and
- c. a polynucleotide selected from the group consisting of SEQ ID NOS:
- 51, 89, 91, 97, 119, 127, 139, 147, 165 and 171;
- and
- d. a polynucleotide which is complementary to the polynucleotide of (a), (b), or (c).
A method of increasing yield in plants, comprising:
- a. introducing into a plant cell a recombinant expression cassette comprising the polynucleotide of claim operably linked to a promoter;
- and
- b. culturing the plant under plant cell growing conditions;
- wherein the plant architecture is improved.
Dependencies are inferred from the claim text.