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US 2008/0178348 A1Published application
Homologous recombination mediated transgene deletion in plant cells
AssigneeMonsanto Technology LLC
InventorLarry A. Gilbertson
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Abstract
A process to prepare a recombined transgenic Zea mays plant or plant cell from a first transgenic Zea mays plant cell, wherein the transgene in the recombinant plant or plant cell has an altered genetic structure relative to the genetic structure of the transgene in the first transgenic plant cell, due to homologous recombination-mediated transgene deletion.
Claims23
A method of preparing a fertile transgenic cell having an altered transgene insertion comprising:
- a) obtaining a first transgenic cell, wherein the transgene insertion DNA sequence comprises an ancillary DNA sequence flanked by directly repeated DNA sequences;
- b) obtaining a plurality of progeny cells of any generation of the first transgenic cell;
- c) selecting a progeny transgenic in the presence of a negative selection agent wherein said ancillary DNA sequence flanked by directly repeated DNA sequences is deleted in the transgene insertion in the progeny transgenic cell as compared to the transgene insertion in the first transgenic cell.
Dependencies are inferred from the claim text.